解脂耶氏酵母赖氨酸分解代谢缺陷型突变体。
Mutants of Saccharomycopsis lipolytica defective in lysine catabolism.
作者信息
Gaillardin C, Fournier P, Sylvestre G, Heslot H
出版信息
J Bacteriol. 1976 Jan;125(1):48-57. doi: 10.1128/jb.125.1.48-57.1976.
Abstract
Wild-type strains of Saccharomycopsis lipolytica are able to use lysine as a carbon or a nitrogen source, but not as a unique source for both. Mutants were selected that could not use lysine either as a nitrogen or as a carbon source. Some of them, however, utilized N-6-acetyllysine or 5-aminovaleric acid. Many of the mutants appeared to be blocked in both utilizations, suggesting a unique pathway for lysine degradation (either as a carbon or as a nitrogen source). Genetic characterization of these mutants was achieved by complementation and recombination tests.
摘要
解脂耶氏酵母的野生型菌株能够将赖氨酸用作碳源或氮源，但不能将其同时用作唯一的碳源和氮源。筛选出了既不能将赖氨酸用作氮源也不能用作碳源的突变体。然而，其中一些突变体能够利用N-6-乙酰赖氨酸或5-氨基戊酸。许多突变体在这两种利用途径上似乎都存在阻断，这表明赖氨酸降解存在一条独特的途径（无论是作为碳源还是氮源）。通过互补和重组试验对这些突变体进行了遗传特征分析。
相似文献
1
Mutants of Saccharomycopsis lipolytica defective in lysine catabolism.解脂耶氏酵母赖氨酸分解代谢缺陷型突变体。
J Bacteriol. 1976 Jan;125(1):48-57. doi: 10.1128/jb.125.1.48-57.1976.
2
Genetic control of lysine permeases in Saccharomycopsis lipolytica.解脂耶氏酵母中赖氨酸通透酶的遗传控制
Arch Microbiol. 1979 Aug 6;122(2):201-5. doi: 10.1007/BF00411361.
3
Mutations affecting simultaneously the lysine and polyphosphates pools in the yeast Saccharomycopsis lipolytica.影响解脂耶氏酵母中赖氨酸和多磷酸盐库的突变。
Biochem Biophys Res Commun. 1981 Jun 16;100(3):1292-8. doi: 10.1016/0006-291x(81)91964-1.
4
Multiple and interconnected pathways for L-lysine catabolism in Pseudomonas putida KT2440.恶臭假单胞菌KT2440中L-赖氨酸分解代谢的多种相互关联途径。
J Bacteriol. 2005 Nov;187(21):7500-10. doi: 10.1128/JB.187.21.7500-7510.2005.
5
Genetic studies on the yeast Saccharomycopsis lipolytica. Inactivation and mutagenesis.解脂耶氏酵母的遗传学研究。失活与诱变。
Z Allg Mikrobiol. 1983;23(3):147-57. doi: 10.1002/jobm.3630230302.
6
Catabolism of lysine in Penicillium chrysogenum leads to formation of 2-aminoadipic acid, a precursor of penicillin biosynthesis.产黄青霉中赖氨酸的分解代谢导致2-氨基己二酸的形成，2-氨基己二酸是青霉素生物合成的前体。
Appl Environ Microbiol. 1994 Jun;60(6):1705-10. doi: 10.1128/aem.60.6.1705-1710.1994.
7
Role of pipecolic acid in the biosynthesis of lysine in Rhodotorula glutinis.哌啶酸在粘红酵母赖氨酸生物合成中的作用。
J Bacteriol. 1979 May;138(2):410-7. doi: 10.1128/jb.138.2.410-417.1979.
8
Lysine catabolism in Rhizoctonia leguminicola and related fungi.豆科丝核菌及相关真菌中的赖氨酸分解代谢
J Bacteriol. 1976 Apr;126(1):338-47. doi: 10.1128/jb.126.1.338-347.1976.
9
Methionine overproduction by Saccharomycopsis lipolytica.解脂耶氏酵母过量生产甲硫氨酸。
Appl Environ Microbiol. 1976 Jul;32(1):125-30. doi: 10.1128/aem.32.1.125-130.1976.
10
Genetic analysis of mating type and alkane utilization in Saccharomycopsis lipolytica.解脂耶氏酵母交配型和烷烃利用的遗传分析。
J Bacteriol. 1973 May;114(2):894-6. doi: 10.1128/jb.114.2.894-896.1973.
引用本文的文献
1
Genetic and biochemical studies of N-alkane non-ultilzing mutants of Saccharomycopsis lipolytica.《解脂假丝酵母 N-链烷烃非利用突变体的遗传和生化研究》
Curr Genet. 1982 Jul;5(2):77-88. doi: 10.1007/BF00365697.
2
Evidence for the control of a mutation in lysine catabolism by the mating type in Yarrowia lipolytica.赖氨酸分解代谢突变受酿酒酵母交配型控制的证据。
Curr Genet. 1984 Sep;8(7):531-6. doi: 10.1007/BF00410440.
3
Characterization of acetyl-CoA: L-lysine N6-acetyltransferase, which catalyses the first step of carbon catabolism from lysine in Saccharomyces cerevisiae.乙酰辅酶A：L-赖氨酸N6-乙酰转移酶的特性，该酶催化酿酒酵母中赖氨酸碳分解代谢的第一步。
Arch Microbiol. 1993;160(5):397-400. doi: 10.1007/BF00252227.
4
LYC1 is the structural gene for lysine N-6-acetyl transferase in yeast.LYC1是酵母中赖氨酸N-6-乙酰转移酶的结构基因。
Curr Genet. 1994 Jan;25(1):24-9. doi: 10.1007/BF00712962.
5
Methionine-defective mutants in Candida utilis.产朊假丝酵母中的甲硫氨酸缺陷型突变体。
Folia Microbiol (Praha). 1981;26(3):184-8. doi: 10.1007/BF02927421.
6
Development of the genetic map of the yeast Saccharomycopsis lipolytica.解脂耶氏酵母遗传图谱的构建。
Mol Gen Genet. 1982;188(2):179-83. doi: 10.1007/BF00332672.
7
Role of L-lysine-alpha-ketoglutarate aminotransferase in catabolism of lysine as a nitrogen source for Rhodotorula glutinis.L-赖氨酸-α-酮戊二酸转氨酶在谷氨酸红酵母利用赖氨酸作为氮源的分解代谢中的作用。
J Bacteriol. 1983 Jul;155(1):417-9. doi: 10.1128/jb.155.1.417-419.1983.
8
Overlapping reading frames at the LYS5 locus in the yeast Yarrowia lipolytica.解脂耶氏酵母中LYS5基因座的重叠阅读框。
Mol Cell Biol. 1990 Sep;10(9):4795-806. doi: 10.1128/mcb.10.9.4795-4806.1990.
9
Characterization of a novel enzyme, N6-acetyl-L-lysine: 2-oxoglutarate aminotransferase, which catalyses the second step of lysine catabolism in Candida maltosa.一种新型酶N6-乙酰-L-赖氨酸：2-酮戊二酸转氨酶的特性，该酶催化麦芽糖假丝酵母中赖氨酸分解代谢的第二步。
Antonie Van Leeuwenhoek. 1992 Nov;62(4):285-90. doi: 10.1007/BF00572596.
10
Physiology of lysine permeases in Saccharomycopsis lipolytica.解脂耶氏酵母中赖氨酸通透酶的生理学
J Bacteriol. 1978 Feb;133(2):492-8. doi: 10.1128/jb.133.2.492-498.1978.
本文引用的文献
1
Metabolic conversion of pipecolic acid to alpha-aminoadipic acid.哌啶酸向α-氨基己二酸的代谢转化。
J Biol Chem. 1962 Sep;237:2828-30.
2
ENZYMIC SYNTHESIS OF EPSILON-N-ACETYL-L-LYSINE.ε-N-乙酰-L-赖氨酸的酶促合成
Arch Biochem Biophys. 1964 Nov;108:221-9. doi: 10.1016/0003-9861(64)90379-0.
3
Deacetylation of alpha-keto-epsilon-acetamidocaproic acid by epsilon-lysine acylase.ε-赖氨酸酰基转移酶催化α-酮-ε-乙酰氨基己酸的脱乙酰化反应。
Biochim Biophys Acta. 1962 Dec 17;65:518-20. doi: 10.1016/0006-3002(62)90459-6.
4
Conversion of alpha-aminoadipic acid to L-pipecolic acid by Aspergillus nidulans.构巢曲霉将α-氨基己二酸转化为L-哌啶酸。
Biochemistry. 1962 Jul;1:606-12. doi: 10.1021/bi00910a010.
5
Studies in lysine biosynthesis. II. Metabolic fate of DL-alpha-aminoadipic acid-6-C14 in T. utilis.赖氨酸生物合成的研究。II. DL-α-氨基己二酸-6-C14在产朊假丝酵母中的代谢命运。
J Biochem. 1961 May;49:392-6. doi: 10.1093/oxfordjournals.jbchem.a127315.
6
The conversion of lysine to pipecolic acid in the rat.大鼠体内赖氨酸向哌啶酸的转化。
J Biol Chem. 1954 Dec;211(2):851-8.
7
The metabolism of lysine in Neurospora.粗糙脉孢菌中赖氨酸的代谢
J Biol Chem. 1954 Dec;211(2):517-29.
8
[Utilization by yeasts of several amino acids as nitrogen source and as nitrogen and carbon sources].[酵母对几种氨基酸作为氮源以及作为氮源和碳源的利用情况]
Ann Inst Pasteur (Paris). 1967 Jul;113(1):81-95.
9
Studies on the metabolism of lysine and 5-hydroxylysine.赖氨酸和5-羟赖氨酸的代谢研究。
Arch Biochem Biophys. 1967 Mar;119(1):336-46. doi: 10.1016/0003-9861(67)90462-6.
10
Diaminopimelate decarboxylase of Lemna perpusilla: partial purification and some properties.浮萍的二氨基庚二酸脱羧酶：部分纯化及某些性质
Biochim Biophys Acta. 1966 May 5;118(2):396-404. doi: 10.1016/s0926-6593(66)80048-6.
Zotero 插件