Dörmer P
J Histochem Cytochem. 1979 Nov;27(11):1529-30. doi: 10.1177/27.11.512339.
Photometric evaluation of autoradiographic grain densities can be performed according to various optical principles. In all instances the amount of light recorded should be a measure of the radioactive substance amount in the specimens. It is shown that grain densities suitable for light microscopic autoradiography are indeed linearly related to radiation exposure. Dependent on the photometric system used, there is a larger or smaller section of linear correlation of the photometric response with grain density. The advantages of incident light bright-field illumination for silver grain counting are discussed. Quantitation of substance amounts in autoradiographs depends on the use of radioactive standard sources. Two different approaches of quantitation are discussed. In 14C-autoradiography which can be applied for cell-kinetic studies, standard plates are used consisting of 14C-polymethylmethacrylate. Allowance has to be made for the different condition of radiation geometry of the cells and the standards. In 125I-autoradiography, 125I-labeled red cells are used as standards. This technique allows for quantitating the number of antibodies bound to individual cell surfaces.
放射自显影片颗粒密度的光度学评估可根据各种光学原理进行。在所有情况下，记录的光量都应是标本中放射性物质含量的一种度量。结果表明，适用于光学显微镜放射自显影的颗粒密度确实与辐射暴露呈线性相关。根据所使用的光度系统，光度响应与颗粒密度的线性相关部分有大有小。文中讨论了入射光亮视野照明用于银颗粒计数的优点。放射自显影片中物质含量的定量取决于放射性标准源的使用。文中讨论了两种不同的定量方法。在可用于细胞动力学研究的¹⁴C放射自显影中，使用由¹⁴C - 聚甲基丙烯酸甲酯组成的标准板。必须考虑细胞和标准物辐射几何条件的差异。在¹²⁵I放射自显影中，使用¹²⁵I标记的红细胞作为标准物。该技术可对结合到单个细胞表面的抗体数量进行定量。
