Keller F, Waser P G
Int J Appl Radiat Isot. 1982 Dec;33(12):1427-32. doi: 10.1016/0020-708x(82)90181-8.
Macroscopic autoradiograms can generally be quantified by exposing previously calibrated standard sources together with the labeled sections and measuring the silver grain density by photometric methods. Variation in section thickness is a real problem with isotopes like 14C, with beta-particles of higher energy than those of 3H. Differences in self-absorption between tissues may be neglected at low section thickness. A simple fitting procedure for non-linear relationship between radiation dose and optical density is described. The combined effect of the examined errors for the practical evaluation of autoradiograms is discussed.
宏观放射自显影片通常可以通过将预先校准的标准源与标记切片一起曝光，并通过光度法测量银粒密度来进行定量。对于像14C这样的同位素，切片厚度的变化是一个实际问题，其β粒子的能量高于3H的β粒子。在低切片厚度下，组织间自吸收的差异可以忽略不计。本文描述了一种用于辐射剂量与光密度之间非线性关系的简单拟合程序。文中讨论了在放射自显影片实际评估中所检测误差的综合影响。
