Vaughan J A, Coombs M E
J Hyg (Lond). 1979 Dec;83(3):521-30. doi: 10.1017/s0022172400026371.
A method is described for the laboratory breeding of the rabbit flea in which the immature stages are reared at constant temperature and humidity. Eggs are obtained by confining fleas taken from a rabbit and her nest shortly after parturition with two of her nestlings in an incubator for 24 h. The eggs are transferred to an artificial diet medium on which the immature stages are reared. On average a female flea produces 50 eggs during the first six days post-partum. At 25 degrees C, 95% of eggs hatched at 79% RH and 98% at 84% RH. Most eggs hatched on the third day after laying and hatching was completed by the fourth day. Significantly more fleas of both sexes were obtained when larvae were reared at 25 degrees C on a medium containing powdered 41B rodent diet than on one containing terrier meal. Both diets also contained yeast and dried rabbit blood. There was no significant difference between the numbers of fleas obtained at 79% RH and 84% RH. Significantly more fleas were also obtained when larvae were reared at 27 degrees C, 84% RH, than at 25 degrees C. Female fleas emerged sooner than males at both 27 degrees C and 25 degrees C. Fleas from the laboratory culture were heavier than those from wild nests. Female fleas were heavier than male fleas in both cases.
本文描述了一种家兔跳蚤实验室繁殖方法，其中未成熟阶段在恒定温度和湿度下饲养。产后不久从兔子及其巢穴中取出跳蚤，与两只幼崽一起置于培养箱中24小时以获取卵。将卵转移至人工饲料培养基上饲养未成熟阶段。平均而言，雌性跳蚤在产后头六天产50枚卵。在25摄氏度时，相对湿度79%时95%的卵孵化，相对湿度84%时98%的卵孵化。大多数卵在产下第三天孵化，第四天孵化完成。当幼虫在25摄氏度下于含有41B啮齿动物粉状饲料的培养基上饲养时，获得的雌雄跳蚤数量显著多于在含有小猎犬粉的培养基上饲养时。两种饲料均含有酵母和干兔血。相对湿度79%和84%时获得的跳蚤数量无显著差异。当幼虫在27摄氏度、相对湿度84%下饲养时，获得的跳蚤数量也显著多于在25摄氏度下饲养时。在27摄氏度和25摄氏度时，雌性跳蚤比雄性跳蚤羽化得早。实验室培养的跳蚤比野外巢穴的跳蚤重。两种情况下雌性跳蚤都比雄性跳蚤重。
