质膜在大鼠肝实质细胞摄取脂肪酸中的作用。
The role of the plasma membrane in fatty acid uptake by rat liver parenchymal cells.
作者信息
Wright J D, Green C
出版信息
Biochem J. 1971 Aug;123(5):837-44. doi: 10.1042/bj1230837.
Abstract
- Suspensions of isolated rat liver parenchymal cells incorporate [(14)C]palmitic acid into glycerides at about 40% of the rate obtained with liver slices. 2. At short time-intervals most of the incorporation is into phosphatidylcholine and this is recovered mainly in the plasma-membrane fraction. 3. At later times (5min to 2h) the [(14)C]palmitic acid is mainly found in triglyceride, but this is not recovered in the plasma-membrane fraction. 4. Addition of lysophosphatidylcholine increases incorporation of palmitic acid into both phosphatidylcholine and triglyceride, with maximum effect at about 0.1mm. 5. In vivo, 1min after injection of [(14)C]palmitic acid, radioactive phosphatidylcholine is concentrated in the plasma-membrane fraction, but the proportion present in this fraction declines rapidly. 6. The phosphatidylcholine of the plasma-membrane fraction has, at 1min after injection, a specific radioactivity 30-fold greater than that of the whole tissue. 7. This phosphatidylcholine reaches its maximum specific radioactivity before the tissue phosphatidic acid or diglyceride. 8. The phosphatidylcholine of the plasma-membrane fraction has a very rapid turnover. 9. It is proposed that the rapid formation of phospholipids in the plasma membrane is by acylation of their lyso-derivatives and the role of this process in fatty acid uptake is discussed.
摘要
- 分离的大鼠肝脏实质细胞悬液将[（14）C]棕榈酸掺入甘油酯的速率约为肝切片的40%。2. 在短时间间隔内，大部分掺入物进入磷脂酰胆碱，且主要在质膜部分中回收。3. 在稍后的时间（5分钟至2小时），[（14）C]棕榈酸主要存在于甘油三酯中，但在质膜部分中未回收。4. 添加溶血磷脂酰胆碱可增加棕榈酸掺入磷脂酰胆碱和甘油三酯的量，在约0.1mM时效果最佳。5. 在体内，注射[（14）C]棕榈酸1分钟后，放射性磷脂酰胆碱集中在质膜部分，但该部分中所占比例迅速下降。6. 在注射后1分钟，质膜部分的磷脂酰胆碱比整个组织的比放射性高30倍。7. 这种磷脂酰胆碱在组织磷脂酸或甘油二酯之前达到其最大比放射性。8. 质膜部分的磷脂酰胆碱周转非常迅速。9. 有人提出，质膜中磷脂的快速形成是通过其溶血衍生物的酰化作用实现的，并讨论了该过程在脂肪酸摄取中的作用。
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