Rothblat G H, Buchko M K
J Lipid Res. 1971 Nov;12(6):647-52.
A number of steroids have been tested in an L-cell tissue culture system to determine their effects on cellular sterol biosynthesis and cellular growth. Cholesterol, desmosterol, lathosterol, 7-dehydrocholesterol, and cholestanone reduce de novo synthesis and produce only limited toxicity at high concentrations of exogenous sterol. Considerable cellular toxicity is observed when cells are grown in the presence of coprostanol and Delta(4)-cholestenone. No marked effect on either cell growth or sterol biosynthesis is produced by cholestanol, beta-sitosterol, stigmasterol, campesterol, ergosterol, cholesteryl oleate, or cholestane.
已在L细胞组织培养系统中对多种类固醇进行了测试，以确定它们对细胞甾醇生物合成和细胞生长的影响。胆固醇、去氢胆固醇、羊毛甾醇、7-脱氢胆固醇和胆甾烷酮可减少从头合成，并且在高浓度外源性甾醇时仅产生有限的毒性。当细胞在粪甾醇和Δ(4)-胆甾烯酮存在的情况下生长时，会观察到相当大的细胞毒性。胆甾醇、β-谷甾醇、豆甾醇、菜油甾醇、麦角甾醇、胆固醇油酸酯或胆甾烷对细胞生长或甾醇生物合成均无明显影响。
