单纯疱疹病毒进入和感染过程中对固醇的分子需求。
Molecular requirement for sterols in herpes simplex virus entry and infectivity.
作者信息
Wudiri George A, Pritchard Suzanne M, Li Hong, Liu Jin, Aguilar Hector C, Gilk Stacey D, Nicola Anthony V
机构信息
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington, USA.
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.
出版信息
J Virol. 2014 Dec;88(23):13918-22. doi: 10.1128/JVI.01615-14. Epub 2014 Sep 17.
Abstract
Herpes simplex virus 1 (HSV-1) required cholesterol or desmosterol for virion-induced membrane fusion. HSV successfully entered DHCR24(-/-) cells, which lack a desmosterol-to-cholesterol conversion enzyme, indicating that entry can occur independently of cholesterol. Depletion of desmosterol from these cells resulted in diminished HSV-1 entry, suggesting a general sterol requirement for HSV-1 entry and that desmosterol can operate in virus entry. Cholesterol functioned more effectively than desmosterol, suggesting that the hydrocarbon tail of cholesterol influences viral entry.
摘要
单纯疱疹病毒1型（HSV-1）的病毒体诱导膜融合需要胆固醇或羊毛甾醇。HSV成功进入缺乏羊毛甾醇向胆固醇转化酶的DHCR24（-/-）细胞，这表明病毒进入可独立于胆固醇发生。从这些细胞中去除羊毛甾醇会导致HSV-1进入减少，这表明HSV-1进入普遍需要甾醇，且羊毛甾醇可在病毒进入过程中发挥作用。胆固醇比羊毛甾醇的作用更有效，这表明胆固醇的烃链影响病毒进入。
相似文献
1
Molecular requirement for sterols in herpes simplex virus entry and infectivity.单纯疱疹病毒进入和感染过程中对固醇的分子需求。
J Virol. 2014 Dec;88(23):13918-22. doi: 10.1128/JVI.01615-14. Epub 2014 Sep 17.
2
Cellular Cholesterol Facilitates the Postentry Replication Cycle of Herpes Simplex Virus 1.细胞胆固醇促进单纯疱疹病毒1的进入后复制周期。
J Virol. 2017 Jun 26;91(14). doi: 10.1128/JVI.00445-17. Print 2017 Jul 15.
3
Herpes Simplex Virus 1 Can Enter Dynamin 1 and 2 Double-Knockout Fibroblasts.单纯疱疹病毒 1 可进入动力蛋白 1 和 2 双敲除成纤维细胞。
J Virol. 2019 Jul 30;93(16). doi: 10.1128/JVI.00704-19. Print 2019 Aug 15.
4
Entry pathways of herpes simplex virus type 1 into human keratinocytes are dynamin- and cholesterol-dependent.单纯疱疹病毒 1 型进入人角质形成细胞的进入途径是网格蛋白和胆固醇依赖性的。
PLoS One. 2011;6(10):e25464. doi: 10.1371/journal.pone.0025464. Epub 2011 Oct 12.
5
Characterization of Vesicular Stomatitis Virus Pseudotypes Bearing Essential Entry Glycoproteins gB, gD, gH, and gL of Herpes Simplex Virus 1.携带单纯疱疹病毒1型主要进入糖蛋白gB、gD、gH和gL的水疱性口炎病毒假型的特性分析
J Virol. 2016 Oct 28;90(22):10321-10328. doi: 10.1128/JVI.01714-16. Print 2016 Nov 15.
6
The amino terminus of herpes simplex virus 1 glycoprotein K is required for virion entry via the paired immunoglobulin-like type-2 receptor alpha.单纯疱疹病毒 1 糖蛋白 K 的氨基末端是通过配对免疫球蛋白样型 2 受体 alpha 进入病毒粒子所必需的。
J Virol. 2013 Mar;87(6):3305-13. doi: 10.1128/JVI.02982-12. Epub 2013 Jan 9.
7
Desmosterol in brain is elevated because DHCR24 needs REST for Robust Expression but REST is poorly expressed.大脑中的羊毛甾醇水平升高，因为7-脱氢胆固醇还原酶24（DHCR24）需要REST来实现稳定表达，但REST的表达水平很低。
Dev Neurosci. 2014;36(2):132-42. doi: 10.1159/000362363. Epub 2014 May 24.
8
Herpes simplex virus internalization into epithelial cells requires Na+/H+ exchangers and p21-activated kinases but neither clathrin- nor caveolin-mediated endocytosis.单纯疱疹病毒内化进入上皮细胞需要钠氢交换体和p21激活激酶，但既不需要网格蛋白介导的内吞作用，也不需要小窝蛋白介导的内吞作用。
J Virol. 2014 Nov;88(22):13378-95. doi: 10.1128/JVI.03631-13. Epub 2014 Sep 10.
9
Functional Characterization of Glycoprotein H Chimeras Composed of Conserved Domains of the Pseudorabies Virus and Herpes Simplex Virus 1 Homologs.由伪狂犬病病毒和单纯疱疹病毒1同源物保守结构域组成的糖蛋白H嵌合体的功能特性
J Virol. 2015 Oct 21;90(1):421-32. doi: 10.1128/JVI.01985-15. Print 2016 Jan 1.
10
A Functional Interaction between Herpes Simplex Virus 1 Glycoprotein gH/gL Domains I and II and gD Is Defined by Using Alphaherpesvirus gH and gL Chimeras.通过使用甲型疱疹病毒gH和gL嵌合体确定单纯疱疹病毒1型糖蛋白gH/gL的结构域I和II与gD之间的功能相互作用。
J Virol. 2015 Jul;89(14):7159-69. doi: 10.1128/JVI.00740-15. Epub 2015 Apr 29.
引用本文的文献
1
Exploring Gene Expression Changes in Murine Female Genital Tract Tissues Following Single and Co-Infection with and Herpes Simplex Virus Type 2.探索小鼠雌性生殖道组织在单纯感染和与2型单纯疱疹病毒共同感染后的基因表达变化。
Pathogens. 2025 Aug 8;14(8):795. doi: 10.3390/pathogens14080795.
2
Viral fusion proteins of classes II and III recognize and reorganize complex biological membranes.II类和III类病毒融合蛋白识别并重组复杂的生物膜。
Commun Biol. 2025 May 9;8(1):717. doi: 10.1038/s42003-025-08040-9.
3
Host Cell Neddylation Facilitates Alphaherpesvirus Entry in a Virus-Specific and Cell-Dependent Manner.宿主细胞的类泛素化作用以病毒特异性和细胞依赖性方式促进α疱疹病毒进入。
Microbiol Spectr. 2022 Oct 26;10(5):e0311422. doi: 10.1128/spectrum.03114-22. Epub 2022 Sep 29.
4
The Cholesterol Transport Inhibitor U18666A Interferes with Pseudorabies Virus Infection.胆固醇转运抑制剂 U18666A 干扰伪狂犬病毒感染。
Viruses. 2022 Jul 14;14(7):1539. doi: 10.3390/v14071539.
5
Cholesterol Biosynthesis Modulates CSFV Replication.胆固醇生物合成调节 CSFV 复制。
Viruses. 2022 Jun 30;14(7):1450. doi: 10.3390/v14071450.
6
Temporal Raman Spectroscopy for Monitoring Replication Kinetics of Epstein-Barr Virus Infection in Glial Cells.用于监测胶质细胞中爱泼斯坦-巴尔病毒感染复制动力学的时间分辨拉曼光谱技术
ACS Omega. 2020 Nov 4;5(45):29547-29560. doi: 10.1021/acsomega.0c04525. eCollection 2020 Nov 17.
7
In Silico Discovery of Candidate Drugs against Covid-19.计算机模拟发现抗新冠病毒候选药物
Viruses. 2020 Apr 6;12(4):404. doi: 10.3390/v12040404.
8
Chewing the Fat: The Conserved Ability of DNA Viruses to Hijack Cellular Lipid Metabolism.闲谈：DNA 病毒劫持细胞脂质代谢的保守能力。
Viruses. 2019 Jan 29;11(2):119. doi: 10.3390/v11020119.
9
The Oxysterol 25-Hydroxycholesterol Inhibits Replication of Murine Norovirus.胆甾醇 25-羟胆固醇抑制鼠诺如病毒的复制。
Viruses. 2019 Jan 24;11(2):97. doi: 10.3390/v11020097.
10
US3 Kinase-Mediated Phosphorylation of Tegument Protein VP8 Plays a Critical Role in the Cellular Localization of VP8 and Its Effect on the Lipid Metabolism of Bovine Herpesvirus 1-Infected Cells.US3 激酶介导的衣壳蛋白 VP8 磷酸化在 VP8 的细胞定位及其对牛疱疹病毒 1 感染细胞脂代谢的影响中起关键作用。
J Virol. 2019 Mar 5;93(6). doi: 10.1128/JVI.02151-18. Print 2019 Mar 15.
本文引用的文献
1
Glycoprotein H and α4β1 integrins determine the entry pathway of alphaherpesviruses.糖蛋白 H 和 α4β1 整合素决定了 α 疱疹病毒的进入途径。
J Virol. 2013 May;87(10):5937-48. doi: 10.1128/JVI.03522-12. Epub 2013 Mar 20.
2
Bacterial colonization of host cells in the absence of cholesterol.在没有胆固醇的情况下，细菌对宿主细胞的定殖。
PLoS Pathog. 2013 Jan;9(1):e1003107. doi: 10.1371/journal.ppat.1003107. Epub 2013 Jan 24.
3
αVβ3-integrin relocalizes nectin1 and routes herpes simplex virus to lipid rafts.αVβ3 整合素使 nectin1 重新分布并将单纯疱疹病毒导向脂筏。
J Virol. 2012 Mar;86(5):2850-5. doi: 10.1128/JVI.06689-11. Epub 2011 Dec 14.
4
Entry pathways of herpes simplex virus type 1 into human keratinocytes are dynamin- and cholesterol-dependent.单纯疱疹病毒 1 型进入人角质形成细胞的进入途径是网格蛋白和胆固醇依赖性的。
PLoS One. 2011;6(10):e25464. doi: 10.1371/journal.pone.0025464. Epub 2011 Oct 12.
5
{alpha}V{beta}3-integrin routes herpes simplex virus to an entry pathway dependent on cholesterol-rich lipid rafts and dynamin2.αVβ3 整合素将单纯疱疹病毒引导至依赖富含胆固醇的脂筏和胞质动力蛋白 2 的进入途径。
Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22260-5. doi: 10.1073/pnas.1014923108. Epub 2010 Dec 6.
6
Reversible conformational change in herpes simplex virus glycoprotein B with fusion-from-without activity is triggered by mildly acidic pH.具有融合外切活性的单纯疱疹病毒糖蛋白 B 的可逆构象变化是由轻度酸性 pH 值触发的。
Virol J. 2010 Dec 1;7:352. doi: 10.1186/1743-422X-7-352.
7
Alphaherpesvirus use and misuse of cellular actin and cholesterol.α疱疹病毒对细胞肌动蛋白和胆固醇的利用和滥用。
Vet Microbiol. 2010 Jun 16;143(1):2-7. doi: 10.1016/j.vetmic.2010.02.007. Epub 2010 Feb 11.
8
Desmosterol can replace cholesterol in sustaining cell proliferation and regulating the SREBP pathway in a sterol-Delta24-reductase-deficient cell line.在一个缺乏甾醇-Δ24-还原酶的细胞系中，去氢胆固醇可以替代胆固醇来维持细胞增殖并调节固醇调节元件结合蛋白（SREBP）途径。
Biochem J. 2009 May 13;420(2):305-15. doi: 10.1042/BJ20081909.
9
Structure-function analysis of herpes simplex virus glycoprotein B with fusion-from-without activity.具有外源性融合活性的单纯疱疹病毒糖蛋白B的结构-功能分析
Virology. 2008 Dec 20;382(2):207-16. doi: 10.1016/j.virol.2008.09.015. Epub 2008 Oct 23.
10
Plasma membrane cholesterol is required for efficient pseudorabies virus entry.高效伪狂犬病病毒进入需要质膜胆固醇。
Virology. 2008 Jul 5;376(2):339-45. doi: 10.1016/j.virol.2008.03.039. Epub 2008 May 8.
Zotero 插件