嗜热菌蛋白酶对含有取代半胱氨酸的二肽衍生物的水解作用。
The hydrolysis by thermolysin of dipeptide derivatives that conatin substituted cysteine.
作者信息
Damoglou A P, Lindley H, Stapleton I W
出版信息
Biochem J. 1971 Jul;123(3):379-84. doi: 10.1042/bj1230379.
Abstract
- The preparation of protected dipeptides of the form acetylglycylamino acid amides is described, where the amino acid is phenylalanine, leucine, valine, alanine, S-methylcysteine, S-ethylcysteine, S-benzylcysteine and S-phenylcysteine. 2. Kinetic parameters for the thermolytic hydrolysis of these blocked dipeptides are reported. The rate of hydrolysis was fastest when the amino acid was leucine or phenylalanine, slower when it was S-methylcysteine, valine or S-ethylcysteine, much slower when it was alanine, and negligible for S-phenylcysteine or S-benzylcysteine. 3. The results are compared with those for similar dipeptide derivatives with benzyloxycarbonyl and furylacryloyl blocking groups, which are hydrolysed faster.
摘要
- 描述了乙酰甘氨酰氨基酸酰胺形式的保护二肽的制备方法，其中氨基酸为苯丙氨酸、亮氨酸、缬氨酸、丙氨酸、S-甲基半胱氨酸、S-乙基半胱氨酸、S-苄基半胱氨酸和S-苯基半胱氨酸。2. 报道了这些封闭二肽热解水解的动力学参数。当氨基酸为亮氨酸或苯丙氨酸时，水解速率最快；当为S-甲基半胱氨酸、缬氨酸或S-乙基半胱氨酸时，水解速率较慢；当为丙氨酸时，水解速率更慢；而对于S-苯基半胱氨酸或S-苄基半胱氨酸，水解速率可忽略不计。3. 将结果与具有苄氧羰基和呋喃丙烯酰封闭基团的类似二肽衍生物的结果进行了比较，后者水解更快。
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