嗜热菌蛋白酶对含有取代半胱氨酸的二肽衍生物的水解作用。
The hydrolysis by thermolysin of dipeptide derivatives that conatin substituted cysteine.
作者信息
Damoglou A P, Lindley H, Stapleton I W
出版信息
Biochem J. 1971 Jul;123(3):379-84. doi: 10.1042/bj1230379.
Abstract
- The preparation of protected dipeptides of the form acetylglycylamino acid amides is described, where the amino acid is phenylalanine, leucine, valine, alanine, S-methylcysteine, S-ethylcysteine, S-benzylcysteine and S-phenylcysteine. 2. Kinetic parameters for the thermolytic hydrolysis of these blocked dipeptides are reported. The rate of hydrolysis was fastest when the amino acid was leucine or phenylalanine, slower when it was S-methylcysteine, valine or S-ethylcysteine, much slower when it was alanine, and negligible for S-phenylcysteine or S-benzylcysteine. 3. The results are compared with those for similar dipeptide derivatives with benzyloxycarbonyl and furylacryloyl blocking groups, which are hydrolysed faster.
摘要
- 描述了乙酰甘氨酰氨基酸酰胺形式的保护二肽的制备方法，其中氨基酸为苯丙氨酸、亮氨酸、缬氨酸、丙氨酸、S-甲基半胱氨酸、S-乙基半胱氨酸、S-苄基半胱氨酸和S-苯基半胱氨酸。2. 报道了这些封闭二肽热解水解的动力学参数。当氨基酸为亮氨酸或苯丙氨酸时，水解速率最快；当为S-甲基半胱氨酸、缬氨酸或S-乙基半胱氨酸时，水解速率较慢；当为丙氨酸时，水解速率更慢；而对于S-苯基半胱氨酸或S-苄基半胱氨酸，水解速率可忽略不计。3. 将结果与具有苄氧羰基和呋喃丙烯酰封闭基团的类似二肽衍生物的结果进行了比较，后者水解更快。
相似文献
1
The hydrolysis by thermolysin of dipeptide derivatives that conatin substituted cysteine.
Biochem J. 1971 Jul;123(3):379-84. doi: 10.1042/bj1230379.
2
[The amino acid composition of the proteins of several varieties of chick-peas and beans grown in Azerbaijan].
Vopr Pitan. 1968 Jul-Aug;27(4):55-8.
3
Effect of amino acid residues at the cleavable site of substrates on the remarkable activation of thermolysin by salts.
Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):133-8. doi: 10.1042/bj3150133.
4
Specificity of Aeromonas aminopeptidase toward amino acid amides and dipeptides.
J Biol Chem. 1972 Feb 25;247(4):1208-10.
5
Nonprotein amino acids from seeds of Cycas circinalis and Phaseolus vulgaris.
Phytochemistry. 1996 May;42(2):443-5. doi: 10.1016/0031-9422(95)00851-9.
6
[Amino acid levels of mare's milk and natural koumiss].
Vopr Pitan. 1974 Sep-Oct;0(5):60-2.
7
8
Inhibition of metalloendopeptidases by 2-mercaptoacetyl-dipeptides.
Eur J Biochem. 1983 Oct 17;136(1):151-4. doi: 10.1111/j.1432-1033.1983.tb07719.x.
9
The specificity of purified porcine pancreatic elastase.
Biochem J. 1969 Aug;114(1):11-7. doi: 10.1042/bj1140011.
10
Effects of normal and their branched alcohols with structurally minimal variation on kinetic parameters in thermolysin-catalyzed peptide hydrolysis and synthesis of N-(benzyloxycarbonyl)-L-phenylalanyl-L-phenylalanine and its methyl ester.
Biosci Biotechnol Biochem. 1995 Mar;59(3):535-7. doi: 10.1271/bbb.59.535.
本文引用的文献
1
A study of the kinetics of the reaction between thiol compounds and choloracetamide.
Biochem J. 1960 Mar;74(3):577-84. doi: 10.1042/bj0740577.
2
SPECIFICITY OF PEPTIDASES. HYDROLYSIS BY TRYPSIN, PAPAIN AND CATHEPSIN B OF SUBSTRATES CONTAINING LYSINE, 4-THIALYSINE OR 4-OXALYSINE.
Biochim Biophys Acta. 1964 Aug 26;89:303-8. doi: 10.1016/0926-6569(64)90218-4.
3
A method for the specific proteolytic cleavage of protein chains.
Arch Biochem Biophys. 1956 Nov;65(1):156-63. doi: 10.1016/0003-9861(56)90184-9.
4
A new synthetic substrate for trypsin and its application to the determination of the amino-acid sequence of proteins.
Nature. 1956 Sep 22;178(4534):647-8. doi: 10.1038/178647a0.
5
The structure of ribonuclease. II. The preparation, separation, and relative alignment of large enzymatically produced fragments.
J Biol Chem. 1956 Jul;221(1):385-404.
6
The specificity of leucine aminopeptidase. III. Action on diastereoisomers.
J Biol Chem. 1952 Dec;199(2):801-17.
7
Observations on the specificity of thermolysin with synthetic peptides.
Biochem Biophys Res Commun. 1966 Aug 12;24(3):427-30. doi: 10.1016/0006-291x(66)90177-x.
8
Thermostable protease from thermophilic bacteria. I. Thermostability, physiocochemical properties, and amino acid composition.
J Biol Chem. 1966 Dec 25;241(24):5919-25.
9
Enzyme specificity: alpha-chymotrypsin.
J Theor Biol. 1965 Sep;9(2):213-28. doi: 10.1016/0022-5193(65)90108-6.
10
The susceptibility of N-acetyl-S-alkyl- and N-acetyl-S-aryl-cysteine ethyl esters to chymotryptic hydrolysis.
Biochem J. 1970 Jul;118(4):553-6. doi: 10.1042/bj1180553.
Zotero 插件