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1
Catalytic activity of -chymotrypsin in which histidine-57 has been methylated.组氨酸-57已被甲基化的α-胰凝乳蛋白酶的催化活性。
Biochem J. 1971 Aug;124(1):13-8. doi: 10.1042/bj1240013.
2
-Chymotrypsin: what can we learn about catalysis from x-ray diffraction?-胰凝乳蛋白酶：从X射线衍射中我们能了解到关于催化作用的哪些信息？
Cold Spring Harb Symp Quant Biol. 1972;36:63-70. doi: 10.1101/sqb.1972.036.01.011.
3
Separation of polar, steric and specific effects in the alpha-chymotrypsin-catalyzed hydrolysis of acyl-substituted p-nitrophenyl esters.α-胰凝乳蛋白酶催化酰基取代对硝基苯酯水解过程中极性、空间效应和特异性效应的分离
Biochem Biophys Res Commun. 1970 Oct 23;41(2):464-70. doi: 10.1016/0006-291x(70)90528-0.
4
THE ROLE OF METHIONINE IN ALPHA-CHYMOTRYPSIN-CATALYSED REACTIONS.甲硫氨酸在α-胰凝乳蛋白酶催化反应中的作用。
Biochem J. 1965 Apr;95(1):180-90. doi: 10.1042/bj0950180.
5
Atypical deacylation of the acyl enzymes formed in the reaction of -chymotrypsin with bis(4-nitrophenyl) carbonate and 0-(4-nitrophenylene) carbonate.在α-胰凝乳蛋白酶与双（4-硝基苯基）碳酸酯和0-（4-亚硝基苯基）碳酸酯反应中形成的酰基酶的非典型脱酰作用。
J Am Chem Soc. 1972 Feb 23;94(4):1316-23. doi: 10.1021/ja00759a044.
6
2-phenylethaneboronic acid, a possible transition-state analog for chymotrypsin.2-苯乙基硼酸，一种可能的胰凝乳蛋白酶过渡态类似物。
Biochemistry. 1971 Jun 22;10(13):2477-83. doi: 10.1021/bi00789a008.
7
[The relationship between the deacylation reaction of acyl-alpha-chymotrypsins--derivatives of aliphatic carbonic acids and temperature].[脂肪族碳酸衍生物酰基-α-糜蛋白酶的脱酰基反应与温度的关系]
Biokhimiia. 1973 Mar-Apr;38(2):381-92.
8
Catalytic activity of dimeric alpha-chymotrypsin. Acylation kinetics at low pH's.二聚体α-胰凝乳蛋白酶的催化活性。低pH值下的酰化动力学。
J Biochem. 1980 Mar;87(3):871-80. doi: 10.1093/oxfordjournals.jbchem.a132817.
9
Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. IV. Pre-steady state kinetic approaches to the investigation of the catalytic hydrolysis of esters.胰凝乳蛋白酶催化特定底物水解的研究。IV. 酯催化水解研究的预稳态动力学方法。
J Biol Chem. 1969 Jul 10;244(13):3483-93.
10
PAPAIN-CATALYSED HYDROLYSIS OF SOME HIPPURIC ESTERS. A NEW MECHANISM FOR PAPAIN-CATALYSED HYDROLYSIS.木瓜蛋白酶催化某些马尿酸酯的水解。木瓜蛋白酶催化水解的新机制。
Biochem J. 1965 Jul;96(1):199-204. doi: 10.1042/bj0960199.

引用本文的文献

1
Mercury(II) binds to both of chymotrypsin's histidines, causing inhibition followed by irreversible denaturation/aggregation.汞（II）与胰凝乳蛋白酶的两个组氨酸结合，导致抑制，随后发生不可逆的变性/聚集。
Protein Sci. 2017 Feb;26(2):292-305. doi: 10.1002/pro.3082. Epub 2017 Jan 14.
2
Transient intermediates in enzymology, 1964-2008.酶学中的瞬态中间体，1964 - 2008年
J Biol Chem. 2015 Apr 24;290(17):10610-26. doi: 10.1074/jbc.X115.650879. Epub 2015 Mar 9.
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Functional dissection of the alphavirus capsid protease: sequence requirements for activity.甲病毒衣壳蛋白酶的功能剖析：活性的序列要求。
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Identification of a potential general acid/base in the reversible phosphoryl transfer reactions catalyzed by tyrosine recombinases: Flp H305.酪氨酸重组酶催化的可逆磷酸基转移反应中潜在的通用酸/碱的鉴定：Flp H305。
Chem Biol. 2007 Feb;14(2):121-9. doi: 10.1016/j.chembiol.2007.01.011.
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Breaking the low barrier hydrogen bond in a serine protease.打破丝氨酸蛋白酶中的低势垒氢键。
Protein Sci. 1999 Feb;8(2):410-7. doi: 10.1110/ps.8.2.410.
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Breaching the conformational integrity of the catalytic triad of the serine protease plasmin: localized disruption of a side chain of His-603 strongly inhibits the amidolytic activity of human plasmin.破坏丝氨酸蛋白酶纤溶酶催化三联体的构象完整性：His-603侧链的局部破坏强烈抑制人纤溶酶的酰胺水解活性。
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5374-7. doi: 10.1073/pnas.90.11.5374.
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Induction of bladder cancer by N-nitroso-dibutylamine and N-nitrososo-butanol-(4)-butylamine.N-亚硝基二丁胺和N-亚硝基丁醇-（4）-丁胺诱发膀胱癌
Br J Cancer. 1972 Dec;26(6):515-6. doi: 10.1038/bjc.1972.72.
8
New developments in enzymatic peptide synthesis.酶促肽合成的新进展。
Experientia. 1991 Dec 1;47(11-12):1123-9. doi: 10.1007/BF01918376.
9
Molecular orbital studies of enzyme activity: catalytic mechanism of serine proteinases.酶活性的分子轨道研究：丝氨酸蛋白酶的催化机制
Proc Natl Acad Sci U S A. 1976 Feb;73(2):432-6. doi: 10.1073/pnas.73.2.432.

本文引用的文献

1
THE ROLE OF METHIONINE IN ALPHA-CHYMOTRYPSIN-CATALYSED REACTIONS.甲硫氨酸在α-胰凝乳蛋白酶催化反应中的作用。
Biochem J. 1965 Apr;95(1):180-90. doi: 10.1042/bj0950180.
2
ACYL INTERMEDIATES IN THE ALPHA-CHYMOTRYPSIN-CATALYZED HYDROLYSIS OF INDOLEACRYLOYLIMIDAZOLE.α-胰凝乳蛋白酶催化吲哚丙烯酰咪唑水解反应中的酰基中间体
J Am Chem Soc. 1965 Apr 20;87:1806-7. doi: 10.1021/ja01086a042.
3
SULFONYL FLUORIDES AS INHIBITORS OF ESTERASES. II. FORMATION AND REACTIONS OF PHENYLMETHANESULFONYL ALPHA-CHYMOTRYPSIN.磺酰氟作为酯酶抑制剂。II. 苯甲磺酰α-胰凝乳蛋白酶的形成与反应。
Biochemistry. 1964 Jun;3:783-91. doi: 10.1021/bi00894a009.
4
Amino acids involved in the action of chymotrypsin.参与胰凝乳蛋白酶作用的氨基酸。
Brookhaven Symp Biol. 1962 Dec;15:101-33.
5
Direct evidence for the presence of histidine in the active center of chymotrypsin.关于组氨酸存在于胰凝乳蛋白酶活性中心的直接证据。
Biochemistry. 1963 Mar-Apr;2:252-5. doi: 10.1021/bi00902a008.
6
Three-dimensional structure of tosyl-alpha-chymotrypsin.甲苯磺酰-α-胰凝乳蛋白酶的三维结构。
Nature. 1967 May 13;214(5089):652-6. doi: 10.1038/214652a0.
7
Optical properties and the chemical nature of acyl-chymotrypsin linkages.酰基-胰凝乳蛋白酶键的光学性质和化学性质
J Am Chem Soc. 1967 May 24;89(11):2726-33. doi: 10.1021/ja00987a040.
8
On the interaction of the active side of alpha-chymotrypsin with chromophores: proflavin binding and enzyme conformation during catalysis.关于α-糜蛋白酶活性位点与发色团的相互作用：催化过程中的原黄素结合与酶构象
J Mol Biol. 1966 Jul;18(3):405-20. doi: 10.1016/s0022-2836(66)80033-5.
9
Implication of an ionizing group in the control of conformation and activity of chymotrypsin.一个电离基团在控制胰凝乳蛋白酶构象和活性中的作用
J Biol Chem. 1966 Jun 10;241(11):2720-30.
10
Modification of alpha-chymotrypsin by methyl p-nitrobenzenesulfonate.对硝基苯磺酸甲酯对α-胰凝乳蛋白酶的修饰作用。
J Am Chem Soc. 1969 Mar 12;91(6):1566-7. doi: 10.1021/ja01034a068.

组氨酸-57已被甲基化的α-胰凝乳蛋白酶的催化活性。

Catalytic activity of -chymotrypsin in which histidine-57 has been methylated.

作者信息

Henderson R

出版信息

Biochem J. 1971 Aug;124(1):13-8. doi: 10.1042/bj1240013.

DOI:10.1042/bj1240013
PMID:5126468
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1177107/
Abstract

The properties of a derivative of alpha-chymotrypsin in which histidine-57 has been methylated have been examined. Although the modified enzyme binds substrate with the same affinity as does native alpha-chymotrypsin, acylation and deacylation occur at much decreased rates. As for native alpha-chymotrypsin, a basic group of pK(a) approx. 7 is involved in both acylation and deacylation. The significance of these results is considered in relation to the normal function of histidine-57.

摘要

已对组氨酸-57被甲基化的α-胰凝乳蛋白酶衍生物的性质进行了研究。尽管修饰后的酶与天然α-胰凝乳蛋白酶以相同的亲和力结合底物，但酰化和脱酰化的速率却大幅降低。与天然α-胰凝乳蛋白酶一样，一个pK(a)约为7的碱性基团参与了酰化和脱酰化过程。结合组氨酸-57的正常功能对这些结果的意义进行了探讨。