Wong C H, Wang K T
Department of Chemistry, Scripps Research Institute, La Jolla, California 92037.
Experientia. 1991 Dec 1;47(11-12):1123-9. doi: 10.1007/BF01918376.
This review article describes new enzymatic methods developed for the efficient and irreversible synthesis of peptides based on native and modified proteases, and for the synthesis of polypeptides containing D- and/or unnatural amino acids. Potential opportunities for future developments in the field based on new enzymes, tailor-made catalytic antibodies, and on the technique of in vitro mutagenesis are also described.
这篇综述文章描述了基于天然和修饰蛋白酶开发的用于高效且不可逆合成肽的新酶法，以及用于合成含D-氨基酸和/或非天然氨基酸的多肽的方法。还描述了基于新酶、定制催化抗体和体外诱变技术在该领域未来发展的潜在机会。
