在乙醇存在的情况下，酸性磷酸酶对核苷酸进行水解和醇解去磷酸化反应。
Hydrolytic and alcoholytic dephosphorylation of nucleotides by acid phosphatase in the presence of ethanol.
作者信息
Tomaszewski M, Buchowicz J
出版信息
Biochem J. 1971 Aug;124(1):189-92. doi: 10.1042/bj1240189.
The effect of ethanol on the activity of acid phosphatase from wheat germ was studied, by using ribonucleoside monophosphates as the enzyme substrates. The nucleotides were effectively degraded to the corresponding nucleosides in the presence of ethanol at all concentrations tested, including a 96% (v/v) solution. However, the nucleotide dephosphorylation was accompanied by the liberation of orthophosphate only when the concentration of ethanol in the assay mixture did not exceed 15%. No inorganic phosphate was liberated when ethanol was present at higher concentrations. Instead, monoethyl phosphate was formed in quantities expected for orthophosphate. The results are explained in terms of phosphatase-catalysed alcoholysis.
以核糖核苷单磷酸作为酶底物，研究了乙醇对小麦胚芽酸性磷酸酶活性的影响。在所有测试浓度的乙醇存在下，包括96%（v/v）的溶液中，核苷酸都能有效地降解为相应的核苷。然而，只有当测定混合物中乙醇浓度不超过15%时，核苷酸去磷酸化才伴随着正磷酸盐的释放。当乙醇浓度较高时，没有释放出无机磷酸盐。相反，形成了预期数量的磷酸单乙酯。这些结果用磷酸酶催化的醇解来解释。
Zotero 插件