Ebihara K, Imamura Y, Kiriyama S
J Nutr. 1979 Dec;109(12):2106-16. doi: 10.1093/jn/109.12.2106.
The effect of dietary mineral composition on the quality of amino acid mixtures and intact casein was examined in growing rats fed for 28 days under "conventional" conditions. When 25% demineralized casein (DC) was the sole dietary protein source, the widely-used mineral mixture by Harper (MMH) supported growth poorly (3.2 g/day), but a new formula mineral mixture (MM2) containing sufficient amounts of zinc gave a growth rate of over 8 g/day for 21 days. With a crude casein (CC) diet, rats grew at the same rate regardless of mineral mixtures. The growth-supporting power of amino acid mixtures, one of which simulated casein (AA-A) and another patterned after Rogers and Harper to give maximal growth of rats (AA-B), was compared with those of DC and CC with addition of MMH or MM2. When the protein source was DC, AA-A or AA-B, MM2 significantly improved the growth over that of rats fed MMH. The growth-stimulating effect of MM2 was greater when added to DC diet than when added to AA-A diet. When MM2 was added to AA-B diet, the growth rate greatly increased and reached that of rats fed DC diet containing MM2 (over 8 g/day), showing a nutritional equivalency between the amino acid mixture and intact protein. Drawbacks inherent in MMH did not appear with CC diet, because CC contained considerable amounts of zinc. When a highly purified amino acid-sucrose diet is used, dietary minerals become most limiting for growth even under conventional conditions. Thus, the previous conflicting results concerning nutritional equivalency of amino acid mixtures and protein can be explained by inadequate mineral sources.
在“常规”条件下对生长大鼠喂养28天，研究了日粮矿物质组成对氨基酸混合物和完整酪蛋白质量的影响。当25%的脱矿质酪蛋白（DC）作为唯一的日粮蛋白质来源时，广泛使用的哈珀矿物质混合物（MMH）对生长的支持作用较差（3.2克/天），但一种含有足够锌的新配方矿物质混合物（MM2）在21天内的生长速率超过8克/天。对于粗酪蛋白（CC）日粮，无论矿物质混合物如何，大鼠的生长速率相同。将氨基酸混合物（其中一种模拟酪蛋白（AA-A），另一种仿照罗杰斯和哈珀的配方以使大鼠生长最大化（AA-B））的生长支持能力与添加了MMH或MM2的DC和CC的生长支持能力进行了比较。当蛋白质来源为DC、AA-A或AA-B时，MM2显著改善了大鼠的生长，超过了喂食MMH的大鼠。MM2添加到DC日粮中的生长刺激作用大于添加到AA-A日粮中的作用。当MM2添加到AA-B日粮中时，生长速率大幅提高，达到喂食含MM2的DC日粮的大鼠的生长速率（超过8克/天），表明氨基酸混合物和完整蛋白质之间具有营养等效性。MMH固有的缺点在CC日粮中未出现，因为CC含有大量的锌。当使用高度纯化的氨基酸-蔗糖日粮时，即使在常规条件下，日粮矿物质对生长也变得最具限制性。因此，先前关于氨基酸混合物和蛋白质营养等效性的相互矛盾的结果可以用矿物质来源不足来解释。
