Shell W E, Kjekshus J K, Sobel B E
J Clin Invest. 1971 Dec;50(12):2614-25. doi: 10.1172/JCI106762.
This study was designed to develop a method for quantitative assessment of infarct size in the conscious animal based on serial changes of serum creatine phosphokinase (CPK) activity. From 11 experiments in which myocardial CPK was injected intravenously in conscious dogs, the average CPK distribution space and average CPK fractional disappearance rate from serum were found to be 11.4% of body weight and 0.48% min respectively. In other experiments, myocardial infarction was produced in 22 conscious dogs by constriction of a left coronary artery snare and serum CPK activity was determined at frequent intervals for 24 hr. Since myocardial CPK depletion reflects infarct size, infarct size was determined directly by analysis of myocardial CPK content in the same animals 24 hr after coronary artery occlusion. CPK released from the infarct was determined from observed changes in serum CPK activity analyzed according to a model taking into account the fraction of CPK released from an infarct and the rates of appearance and disappearance of CPK activity from serum. Infarct size was calculated on the basis of observed changes in serum CPK and compared to infarct size determined directly by analysis of myocardial CPK depletion. Agreement was close and results from all experiments fit the equation: [infarct size (g) determined from serum CPK] = 1.13 x [infarct size (g) determined from myocardial CPK] - 1.3, r = 0.96, n = 22. The method described is useful for accurate assessment of infarct size in the conscious animal and for detection of modification of infarct size produced by pharmacologic interventions.
本研究旨在开发一种基于血清肌酸磷酸激酶（CPK）活性的系列变化对清醒动物梗死面积进行定量评估的方法。在11项向清醒犬静脉注射心肌CPK的实验中，发现平均CPK分布空间和血清中CPK的平均分数消失率分别为体重的11.4%和0.48%/分钟。在其他实验中，通过结扎左冠状动脉在22只清醒犬中造成心肌梗死，并在24小时内频繁测定血清CPK活性。由于心肌CPK的消耗反映梗死面积，在冠状动脉闭塞24小时后，通过分析同一动物的心肌CPK含量直接确定梗死面积。根据一个考虑梗死释放的CPK分数以及血清中CPK活性出现和消失速率的模型，从观察到的血清CPK活性变化中确定梗死释放的CPK。根据观察到的血清CPK变化计算梗死面积，并与通过分析心肌CPK消耗直接确定的梗死面积进行比较。两者吻合度很高，所有实验结果均符合以下方程：[由血清CPK确定的梗死面积（g）]=1.13×[由心肌CPK确定的梗死面积（g）]-1.3，r=0.96，n=22。所描述的方法对于准确评估清醒动物的梗死面积以及检测药物干预引起的梗死面积变化很有用。
