Gerone P J, Couch R B, Knight V
Appl Microbiol. 1971 Nov;22(5):899-903. doi: 10.1128/am.22.5.899-903.1971.
The performance of an aerosol inoculator for human volunteers is described in tests that used the PR8 strain of type A influenza virus and sodium fluorescein as a physical tracer. Virus recovery from the aerosols was approximately 1% and was unaffected by such variables as prolonged aerosolization, total airflow, relative humidity, or method of sampling. The recovery of sodium fluorescein from the aerosol was approximately 12% and was influenced by total airflow rates and relative humidity. With this apparatus, it should be possible to deliver reasonably predictable and measurable doses of respiratory viruses to human subjects. The design makes it possible to dismantle the inoculator into its component parts to facilitate portability.
在使用甲型流感病毒PR8株和荧光素钠作为物理示踪剂的试验中，描述了一种用于人类志愿者的气溶胶接种器的性能。从气溶胶中回收的病毒约为1%，不受雾化时间延长、总气流、相对湿度或采样方法等变量的影响。从气溶胶中回收的荧光素钠约为12%，受总气流速率和相对湿度的影响。使用该装置，应该能够向人类受试者输送合理可预测和可测量剂量的呼吸道病毒。该设计使得可以将接种器拆解成其组成部件，以方便携带。
