Karim Y G, Ijaz M K, Sattar S A, Johnson-Lussenburg C M
Can J Microbiol. 1985 Nov;31(11):1058-61. doi: 10.1139/m85-199.
Rhinovirus-14, suspended in tryptose phosphate broth supplemented with uranine (physical tracer) and an antifoam, was aerosolized by use of a Collison nebulizer. The aerosols were held in a rotating drum with the relative humidity at either the low (30 +/- 5%), medium (50 +/- 5%), or high (80 +/- 5%) level at 20 +/- 1 degrees C. An all-glass impinger was used to recover the virus from the air in the drum, with the first air sample being collected after a 15-min period of aerosol stabilization. Subsequent air samples were withdrawn at 2, 4, 8, and 14 h after stabilization of the aerosol. At the low and medium relative humidity levels, the infectivity of the airborne virus was rapidly lost and less than 0.25% could be detected in the first air sample. At the high RH level, however, the airborne virus had a half-life of 13.7 +/- 1.91 h and nearly 30% of the input infectious virus could be detected in the drum air even after 24 h of aerosolization. These findings suggest that under certain environmental conditions, notably high relative humidity, air may act as a vehicle for the spread of rhinovirus infections.
将14型鼻病毒悬浮于添加了尿酸（物理示踪剂）和消泡剂的胰蛋白胨磷酸盐肉汤中，使用科里森雾化器将其雾化。气溶胶被置于一个转鼓中，在20±1摄氏度下，相对湿度保持在低（30±5%）、中（50±5%）或高（80±5%）水平。使用全玻璃撞击式采样器从转鼓中的空气中回收病毒，在气溶胶稳定15分钟后采集第一个空气样本。在气溶胶稳定后2、4、8和14小时抽取后续空气样本。在低和中相对湿度水平下，空气中病毒的传染性迅速丧失，在第一个空气样本中检测到的病毒不到0.25%。然而，在高相对湿度水平下，空气中病毒的半衰期为13.7±1.91小时，即使在雾化24小时后，转鼓空气中仍能检测到近30%的输入感染性病毒。这些发现表明，在某些环境条件下，特别是高相对湿度下，空气可能成为鼻病毒感染传播的媒介。
