Miyaki M, Akamatsu N, Ono T, Koyama H
Mutat Res. 1979 Nov;68(3):259-63. doi: 10.1016/0165-1218(79)90157-5.
The mutations at the hypoxanthine-guanine phosphoribosyl transferase (HGPRTase) locus in Chinese hamster V79 cells induced by metal cations were examined by the development of resistance to 8-azaguanine (8AG). The spontaneous frequency of 8AG resistance was 5.8 per 10(6) cells, and the frequency was enhanced to 2-6 times that of the control by treatment of cells with the chlorides of beryllium and manganese. About 75% of 8AG-resistant colonies were sensitive to amethopterin, and 86% of the resistant colonies showed less than 3% of the HGPRTase activity of the wild-type cells. The mutant frequency in cultures treated with cobalt and nickel chlorides were slightly increased, and mutation induction was only detectable at very low rates of cell survival.
通过对8-氮杂鸟嘌呤（8AG）抗性的发展来检测金属阳离子诱导的中国仓鼠V79细胞次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRTase）基因座的突变。8AG抗性的自发频率为每10⁶个细胞5.8个，用铍和锰的氯化物处理细胞后，该频率提高到对照的2至6倍。约75%的8AG抗性菌落对氨甲蝶呤敏感，86%的抗性菌落显示的HGPRTase活性低于野生型细胞的3%。用钴和镍的氯化物处理的培养物中的突变频率略有增加，并且仅在细胞存活率非常低的情况下才能检测到突变诱导。
