Helman M, Givol D
Biochem J. 1971 Dec;125(4):971-4. doi: 10.1042/bj1250971.
Antibodies to nitrotyrosine were prepared in goats or rabbits by injecting a nitrotyrosine-protein conjugate. The antibodies were purified by using a protein that had been nitrated with tetranitromethane. These antibodies were used to isolate nitrotyrosine-containing peptides from nitrated lysozyme. The nitrotyrosine-containing peptides were thus purified (55% yield) in one step and the positions of nitration in lysozyme were found to be at tyrosine-20 and tyrosine-23. This method is of general applicability for the determination of the position of nitrotyrosine in proteins.
通过注射硝基酪氨酸 - 蛋白质偶联物，在山羊或兔子体内制备抗硝基酪氨酸抗体。使用经四硝基甲烷硝化的蛋白质对抗体进行纯化。这些抗体用于从硝化溶菌酶中分离含硝基酪氨酸的肽段。含硝基酪氨酸的肽段由此一步纯化（产率55%），并发现溶菌酶中的硝化位点位于酪氨酸 - 20和酪氨酸 - 23处。该方法对于确定蛋白质中硝基酪氨酸的位置具有普遍适用性。
