微生物蛋白酶和动物蛋白酶对琼脂凝胶中肽-蛋白质-木质素磺酸复合物的影响。
The effect of microbial and animal proteinases on peptide- and protein-lignosulphonic acid complexes in agar gel.
作者信息
Naess B
出版信息
Acta Vet Scand. 1971;12(4):592-600. doi: 10.1186/BF03547727.
Abstract
It is shown that various microbial and animal proteinases, with the exception of pepsin, have a lytic effect on peptide- and protein-lignosulphonic acid complexes in agar gel, and that this effect is characteristically inhibited by specific antiproteinases and naturally occurring serum inhibitors. The activity of the enzymes is studied at various concentrations of peptides and proteins, and at various pH values in the agar. The lysis phenomenon is seen in relation to the precipitation theory of peptide- and protein-lignosulphonic acid complexes, and to biochemical reactions taking place in certain natural ecosystems. Activity caused by different microbial proteinases is demonstrated in agar plates at pH 3.6. The possibility of using the method for studies on proteolytic enzymes at low pH values is suggested.
摘要
结果表明，除胃蛋白酶外，各种微生物蛋白酶和动物蛋白酶对琼脂凝胶中的肽 - 蛋白质 - 木质素磺酸复合物具有溶解作用，且这种作用具有特征性地被特异性抗蛋白酶和天然存在的血清抑制剂所抑制。在琼脂中不同浓度的肽和蛋白质以及不同pH值下研究了这些酶的活性。溶解现象与肽 - 蛋白质 - 木质素磺酸复合物的沉淀理论以及某些自然生态系统中发生的生化反应有关。在pH 3.6的琼脂平板上证明了不同微生物蛋白酶引起的活性。提出了使用该方法研究低pH值下蛋白水解酶的可能性。
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引用本文的文献
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The effects of peptide-precipitating lignosulphonic acids on the in vitro proteolytic activity of some animal and microbial proteinases.肽沉淀木质素磺酸对某些动物和微生物蛋白酶体外蛋白水解活性的影响。
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2
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Acta Vet Scand. 1973;14(1):129-46. doi: 10.1186/BF03547416.
3
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4
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本文引用的文献
1
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Acta Vet Scand. 1971;12(4):583-91. doi: 10.1186/BF03547726.
2
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Acta Vet Scand. 1971;12(4):572-82. doi: 10.1186/BF03547725.
3
Proteolytic enzymes and biological inhibitors. IV. Bacterial proteinase inhibitors and their effect upon enzymes of various origin.
Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(6):755-9.
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Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(3):350-62.
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