Feherty P, Farrer-Brown G, Kellie A E
Br J Cancer. 1971 Dec;25(4):697-710. doi: 10.1038/bjc.1971.85.
An assay is described for measuring the concentration of specific, high-affinity oestradiol receptors in the cell supernatant fraction of breast tumour biopsies. The method has been applied to biopsies from 94 patients with malignant and benign diseases of the breast. Of the 53 biopsies classified as carcinomas, 37 contained high-affinity oestradiol receptors in concentrations ranging from 0·3-22·6 × 10(-15) moles/mg. tissue, 2 were borderline, and 14 did not contain any receptor. The proportion of positive results and the range of concentrations were found to be somewhat higher in postmenopausal than in premenopausal patients. Despite detailed examination, no histological feature was found which could explain the variation in receptor concentration; neither could it be accounted for by differences in the cellularity of the biopsies. Of the 41 benign breast biopsies examined only 3 contained any high-affinity oestradiol receptor and in these the concentration was very low, ranging from 0·3-0·6 × 10(-15) moles/mg. tissue. The receptor has not been detected in normal breast tissue. The relationship between the presence of oestrogen receptors and hormone responsiveness in tumours is discussed.
本文描述了一种用于测量乳腺肿瘤活检细胞上清液中特异性高亲和力雌二醇受体浓度的检测方法。该方法已应用于94例患有乳腺良恶性疾病患者的活检样本。在53例被分类为癌的活检样本中，37例含有高亲和力雌二醇受体，其浓度范围为0·3 - 22·6×10(-15)摩尔/毫克组织，2例处于临界状态，14例未含有任何受体。结果发现，绝经后患者的阳性结果比例和浓度范围比绝经前患者略高。尽管进行了详细检查，但未发现任何组织学特征能够解释受体浓度的差异；活检样本细胞密度的差异也无法解释这种差异。在41例接受检查的乳腺良性活检样本中，只有3例含有任何高亲和力雌二醇受体，且其浓度非常低，范围为0·3 - 0·6×10(-15)摩尔/毫克组织。在正常乳腺组织中未检测到该受体。本文还讨论了雌激素受体的存在与肿瘤激素反应性之间的关系。
