人胎盘胆碱乙酰转移酶的动力学特性
The kinetic properties of human placental choline acetyltransferase.
作者信息
Morris D, Maneckjee A, Hebb C
出版信息
Biochem J. 1971 Dec;125(3):857-63. doi: 10.1042/bj1250857.
Abstract
- Michaelis constants for human placental choline acetyltransferase were shown to be dependent on the concentration of the second substrate present. The primary plots indicate a sequential rather than a Ping Pong mechanism and are of the same type with 300mm- and 500mm-sodium chloride. 2. Similar results have been obtained with rabbit brain choline acetyltransferase. 3. Product inhibition of the forward reaction has been studied. CoA inhibits competitively with respect to acetyl-CoA and non-competitively with respect to choline. Acetylcholine inhibits competitively with respect to choline and non-competitively with respect to acetyl-CoA. No inhibition is given by acetylcholine when the enzyme is saturated with choline. 4. It is concluded that human placental choline acetyltransferase has an ordered mechanism of the Theorell-Chance type.
摘要
- 已表明人胎盘胆碱乙酰转移酶的米氏常数取决于存在的第二种底物的浓度。初始图表明是顺序机制而非乒乓机制，并且在300mM和500mM氯化钠条件下是同一类型。2. 兔脑胆碱乙酰转移酶也得到了类似结果。3. 对正向反应的产物抑制进行了研究。辅酶A对乙酰辅酶A呈竞争性抑制，对胆碱呈非竞争性抑制。乙酰胆碱对胆碱呈竞争性抑制，对乙酰辅酶A呈非竞争性抑制。当酶被胆碱饱和时，乙酰胆碱无抑制作用。4. 得出结论：人胎盘胆碱乙酰转移酶具有Theorell-Chance类型的有序机制。
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本文引用的文献
1
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J Physiol. 1952 Jun;117(2):234-40. doi: 10.1113/jphysiol.1952.sp004743.
2
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J Neurochem. 1965 May;12:363-72. doi: 10.1111/j.1471-4159.1965.tb04237.x.
3
EQUILIBRIUM REACTION RATES AND THE MECHANISMS OF BOVINE HEART AND RABBIT MUSCLE LACTATE DEHYDROGENASES.牛心和兔肌乳酸脱氢酶的平衡反应速率及作用机制
J Biol Chem. 1964 Nov;239:3901-7.
4
A COMPARISON OF THE N-ALKYL GROUP SPECIFICITY OF CHOLINE ACETYLTRANSFERASE FROM DIFFERENT SPECIES.不同物种胆碱乙酰转移酶的N-烷基基团特异性比较
J Neurochem. 1964 Nov;11:793-803. doi: 10.1111/j.1471-4159.1964.tb06728.x.
5
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6
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7
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