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人胎盘胆碱乙酰转移酶的动力学特性

The kinetic properties of human placental choline acetyltransferase.

作者信息

Morris D, Maneckjee A, Hebb C

出版信息

Biochem J. 1971 Dec;125(3):857-63. doi: 10.1042/bj1250857.

DOI:10.1042/bj1250857
PMID:5145909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1178191/
Abstract
  1. Michaelis constants for human placental choline acetyltransferase were shown to be dependent on the concentration of the second substrate present. The primary plots indicate a sequential rather than a Ping Pong mechanism and are of the same type with 300mm- and 500mm-sodium chloride. 2. Similar results have been obtained with rabbit brain choline acetyltransferase. 3. Product inhibition of the forward reaction has been studied. CoA inhibits competitively with respect to acetyl-CoA and non-competitively with respect to choline. Acetylcholine inhibits competitively with respect to choline and non-competitively with respect to acetyl-CoA. No inhibition is given by acetylcholine when the enzyme is saturated with choline. 4. It is concluded that human placental choline acetyltransferase has an ordered mechanism of the Theorell-Chance type.
摘要
  1. 已表明人胎盘胆碱乙酰转移酶的米氏常数取决于存在的第二种底物的浓度。初始图表明是顺序机制而非乒乓机制，并且在300mM和500mM氯化钠条件下是同一类型。2. 兔脑胆碱乙酰转移酶也得到了类似结果。3. 对正向反应的产物抑制进行了研究。辅酶A对乙酰辅酶A呈竞争性抑制，对胆碱呈非竞争性抑制。乙酰胆碱对胆碱呈竞争性抑制，对乙酰辅酶A呈非竞争性抑制。当酶被胆碱饱和时，乙酰胆碱无抑制作用。4. 得出结论：人胎盘胆碱乙酰转移酶具有Theorell-Chance类型的有序机制。

相似文献

1
The kinetic properties of human placental choline acetyltransferase.人胎盘胆碱乙酰转移酶的动力学特性
Biochem J. 1971 Dec;125(3):857-63. doi: 10.1042/bj1250857.
2
Re-evaluation of the kinetic mechanism of the choline acetyltransferase reaction.胆碱乙酰转移酶反应动力学机制的重新评估。
J Biol Chem. 1977 Jul 25;252(14):4796-802.
3
The kinetic properties of human placental choline acetyltransferase.人胎盘胆碱乙酰转移酶的动力学特性
Biochem J. 1971 Jan;121(1):20P-21P. doi: 10.1042/bj1210020pb.
4
Kinetic mechanisms of human placental choline acetyltransferase.人胎盘胆碱乙酰转移酶的动力学机制
Biochem Pharmacol. 1972 Mar 15;21(6):787-802. doi: 10.1016/0006-2952(72)90122-0.
5
The use of choline acetyltransferase for measuring the synthesis of acetyl-coenzyme A and its release from brain mitochondria.使用胆碱乙酰转移酶来测量乙酰辅酶A的合成及其从脑线粒体中的释放。
Biochem J. 1967 Sep;104(3):749-56. doi: 10.1042/bj1040749.
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Isotopic exchange between acetylcholine and [Me-14C] choline catalysed by human placental choline acetyltransferase.人胎盘胆碱乙酰转移酶催化的乙酰胆碱与[甲基-14C]胆碱之间的同位素交换
Biochem J. 1969 Oct;114(4):85P. doi: 10.1042/bj1140085pa.
7
Human placental choline acetyltransferase. Radiometric assay, inhibition by analogues of choline and acetylcholine, and isotopic exchange between choline and acetylcholine.人胎盘胆碱乙酰转移酶。放射性测定、胆碱和乙酰胆碱类似物的抑制作用以及胆碱与乙酰胆碱之间的同位素交换。
Eur J Biochem. 1971 Oct 26;22(4):563-72. doi: 10.1111/j.1432-1033.1971.tb01578.x.
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Control of acetylcholine synthesis--the inhibition of choline acetyltransferase by acetylcholine.乙酰胆碱合成的调控——乙酰胆碱对胆碱乙酰转移酶的抑制作用。
J Neurochem. 1969 Jul;16(7):1185-91. doi: 10.1111/j.1471-4159.1969.tb05964.x.
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Some kinetic studies on the mechanism of action of carnitine acetyltransferase.关于肉碱乙酰转移酶作用机制的一些动力学研究。
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10
Effect of environmental temperature on the kinetic properties of goldfish brain choline acetyltransferase.环境温度对金鱼脑胆碱乙酰转移酶动力学特性的影响。
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引用本文的文献

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Exercise enhances motor skill learning by neurotransmitter switching in the adult midbrain.运动通过成年中脑的神经递质转换来增强运动技能学习。
Nat Commun. 2020 May 4;11(1):2195. doi: 10.1038/s41467-020-16053-7.
2
Studies on choline acetyltransferase isolated from human brain.从人脑中分离出的胆碱乙酰转移酶的研究。
Neurochem Res. 1977 Jun;2(3):281-91. doi: 10.1007/BF00969358.
3
Some properties of choline acetyltransferase isolated from the electric organ ofTorpedo marmorata: Specificity for choline analogues and inhibition by certain amines and amino acids.从电鳐电器官中分离出的胆碱乙酰转移酶的某些性质：对胆碱类似物的特异性和某些胺类及氨基酸的抑制作用。
Neurochem Res. 1976 Apr;1(2):153-69. doi: 10.1007/BF00966107.
4
Evidence for presence of an arginine residue in the coenzyme A binding site of choline acetyltransferase.胆碱乙酰转移酶辅酶A结合位点存在精氨酸残基的证据。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7449-52. doi: 10.1073/pnas.78.12.7449.
5
The synthesis of acetylcholine by plants.植物中乙酰胆碱的合成。
Biochem J. 1981 Jan 15;194(1):361-4. doi: 10.1042/bj1940361.
6
Effect of environmental temperature on the kinetic properties of goldfish brain choline acetyltransferase.环境温度对金鱼脑胆碱乙酰转移酶动力学特性的影响。
Biochem J. 1972 Oct;129(5):1013-21. doi: 10.1042/bj1291013.
7
The reaction of acetyl-coenzyme A with choline acetyltransferase.乙酰辅酶A与胆碱乙酰转移酶的反应。
Biochem J. 1972 Oct;129(4):991-4. doi: 10.1042/bj1290991.
8
The storage and release of acetylcholine.乙酰胆碱的储存与释放。
Biochem J. 1972 Jul;128(3):73P-74P. doi: 10.1042/bj1280073p.
9
On the mechanism of action of choline acetyltransferase.论胆碱乙酰转移酶的作用机制。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3355-8. doi: 10.1073/pnas.71.9.3355.
10
The purification of choline acetyltransferase of squid-head ganglia.鱿鱼头部神经节胆碱乙酰转移酶的纯化
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3749-53. doi: 10.1073/pnas.70.12.3749.

本文引用的文献

1
The level of free choline in plasma.血浆中游离胆碱的水平。
J Physiol. 1952 Jun;117(2):234-40. doi: 10.1113/jphysiol.1952.sp004743.
2
THE MORPHOLOGY AND ACETYLCHOLINE CONTENT OF ISOLATED CEREBRAL CORTICAL SYNAPTIC VESICLES.分离的大脑皮质突触小泡的形态学与乙酰胆碱含量
J Neurochem. 1965 May;12:363-72. doi: 10.1111/j.1471-4159.1965.tb04237.x.
3
EQUILIBRIUM REACTION RATES AND THE MECHANISMS OF BOVINE HEART AND RABBIT MUSCLE LACTATE DEHYDROGENASES.牛心和兔肌乳酸脱氢酶的平衡反应速率及作用机制
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4
A COMPARISON OF THE N-ALKYL GROUP SPECIFICITY OF CHOLINE ACETYLTRANSFERASE FROM DIFFERENT SPECIES.不同物种胆碱乙酰转移酶的N-烷基基团特异性比较
J Neurochem. 1964 Nov;11:793-803. doi: 10.1111/j.1471-4159.1964.tb06728.x.
5
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations.具有两种或更多种底物或产物的酶催化反应动力学。I. 命名法和速率方程。
Biochim Biophys Acta. 1963 Jan 8;67:104-37. doi: 10.1016/0006-3002(63)91800-6.
6
A new and rapid colorimetric determination of acetylcholinesterase activity.一种新的快速比色法测定乙酰胆碱酯酶活性。
Biochem Pharmacol. 1961 Jul;7:88-95. doi: 10.1016/0006-2952(61)90145-9.
7
The substrate specificity of carnitine acetyltransferase.肉碱乙酰转移酶的底物特异性。
Biochem J. 1967 Aug;104(2):510-8. doi: 10.1042/bj1040510.
8
Control of acetylcholine synthesis--the inhibition of choline acetyltransferase by acetylcholine.乙酰胆碱合成的调控——乙酰胆碱对胆碱乙酰转移酶的抑制作用。
J Neurochem. 1969 Jul;16(7):1185-91. doi: 10.1111/j.1471-4159.1969.tb05964.x.
9
Choline acetyltransferase from rat brain.来自大鼠大脑的胆碱乙酰转移酶。
J Biol Chem. 1968 Jul 25;243(14):3864-70.
10
Purification and properties of choline acetyltransferase from ox brain striate nuclei.牛脑纹状体核中胆碱乙酰转移酶的纯化及性质
J Neurochem. 1971 Apr;18(4):571-80. doi: 10.1111/j.1471-4159.1971.tb11987.x.