Emmrich F, Storch W, von Baehr R, Schwenke H
Z Gesamte Inn Med. 1975 Apr 15;30(8):302-6, 307.
Isolated lymphocytes from peripheral blood of 22 healthy donors were examined with the help of the indirect membrane immunofluorescence (MIF) for immunoglobulin determinants on the surface of the cells. The quantity of MIF+-cells for IgM was on the average 8.7% (3-18) and for IgG 15.5%(8-18). Five different anti-IgM-sera were characterised with regard to their use in the MIF (precipitation titre, entire protein content, content of anti-IgM-antibodies). Differences in the quantity of the MIF+-lymphocytes were above all based on differing antibody concentrations of the sera. It was referred to further possible causes for these differences. The influence on the results of the examinations by protein addition and trypsination.
借助间接膜免疫荧光法（MIF），对22名健康供体外周血中分离出的淋巴细胞进行检测，以分析细胞表面免疫球蛋白的决定簇。IgM的MIF+细胞平均数量为8.7%（3%-18%），IgG的为15.5%（8%-18%）。针对五种不同的抗IgM血清在MIF中的应用进行了特性分析（沉淀效价、总蛋白含量、抗IgM抗体含量）。MIF+淋巴细胞数量的差异主要基于血清中抗体浓度的不同。文中还提及了造成这些差异的其他可能原因，以及蛋白质添加和胰蛋白酶处理对检测结果的影响。
