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正常和感染的光滑双脐螺的组织化学与电泳研究。II. 酸性和碱性磷酸酶

Histochemical and electrophoretical studies in normal and infected Biomphalaria glabrata. II. Acid and alkaline phosphatase.

作者信息

Dardenne M A, Danni T M, Narang N, Narang S

出版信息

Rev Bras Pesqui Med Biol. 1979 Jun;12(2-3):133-9.

PMID:515466
Abstract

Various organs of normal and infected Biomphalaria glabrata were analysed, electrophoretically and histochemically, with respect to the acid phosphatase and alkaline phosphatase activity. In general, digestive gland, ovotestis and stomach show an increase in acid phosphatase activity in infected snails, whereas kidney and albumen gland show less activity. Alkaline phosphatase does not show any significant different in activity between normal and infected snails. Electrophoretically digestive glands of snails, twenty days after infection, show an extra faint band, whereas seven days after infection, the snails do not show any such extra band. Histochemically also after twenty days, infected snails show more activity in the sporocyst region, but those infected for only seven days, do not show any increase in acid phosphatase activity.

摘要

对正常和受感染的光滑双脐螺的各种器官进行了电泳和组织化学分析,以检测酸性磷酸酶和碱性磷酸酶的活性。总体而言,受感染蜗牛的消化腺、卵睾和胃中酸性磷酸酶活性增加,而肾脏和蛋白腺的活性较低。正常蜗牛和受感染蜗牛之间碱性磷酸酶的活性没有显著差异。电泳分析显示,感染后20天的蜗牛消化腺出现一条额外的 faint 带,而感染后7天的蜗牛没有出现任何此类额外带。组织化学分析也表明,感染20天后,受感染蜗牛的包囊区域活性更高,但仅感染7天的蜗牛酸性磷酸酶活性没有增加。

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