Gribanov G A, Shchennikova V V
Vopr Med Khim. 1983 May-Jun;29(3):53-7.
Alterations in activity of acid and alkaline phosphatases as well as in concentration of inorganic phosphate were studied in tissue and homogenates of adrenal glands and testicles of rats within 48 hrs of the postmortal autolysis. During the autolysis acid phosphatase of homogenate and tissue of adrenal glands as well as alkaline phosphatase from the gland were more stable than acid and alkaline phosphatases from testicles. Activities of acid and alkaline phosphatases decreased gradually in homogenate and tissue of testicles during autolysis. Within the first day of autolysis distinct inhibition of alkaline phosphatase was also observed in the homogenate of testicles and adrenal glands. An increase in amount of inorganic phosphate in tissue and homogenate of adrenal gland and in tissue of testicles did not depend on alterations in activity of the phosphatases on autolysis. Prehomogenization of these tissues altered markedly the enzymatic activity both in adrenal glands and in testicles.
在大鼠死后自溶的48小时内,对肾上腺和睾丸组织及匀浆中酸性和碱性磷酸酶的活性以及无机磷酸盐浓度的变化进行了研究。在自溶过程中,肾上腺匀浆和组织中的酸性磷酸酶以及肾上腺中的碱性磷酸酶比睾丸中的酸性和碱性磷酸酶更稳定。睾丸匀浆和组织中的酸性和碱性磷酸酶活性在自溶过程中逐渐降低。在自溶的第一天,在睾丸和肾上腺匀浆中也观察到碱性磷酸酶有明显抑制。肾上腺组织和匀浆以及睾丸组织中无机磷酸盐量的增加并不取决于自溶时磷酸酶活性的变化。这些组织的预匀浆显著改变了肾上腺和睾丸中的酶活性。