McCarthy C
Infect Immun. 1971 Sep;4(3):199-204. doi: 10.1128/iai.4.3.199-204.1971.
Mycobacterium avium accumulates (14)C-palmitic acid with saturation kinetics; the process is both temperature dependent and pH sensitive. The fatty acid is incorporated into triglyceride in vivo and the conversion is detectable within 5 min after exposure of the cells to (14)C-palmitic acid. The triglyceride is rapidly utilized because (14)CO(2) evolution from it begins within 30 min after (14)C-palmitic acid accumulation. Data from silicic acid column chromatography of extracts of cultures that have divided many times in medium containing (14)C-palmitic acid indicate that a large proportion of the cell lipid is triglyceride, but the radioactivity is widely dispersed among the other lipids. It is estimated that about 5% of the cell dry weight is triglyceride in a postexponential culture.
鸟分枝杆菌以饱和动力学方式积累¹⁴C-棕榈酸；该过程既依赖温度又对pH敏感。脂肪酸在体内被掺入甘油三酯中，并且在细胞暴露于¹⁴C-棕榈酸后5分钟内即可检测到这种转化。甘油三酯被迅速利用，因为在¹⁴C-棕榈酸积累后30分钟内就开始有¹⁴CO₂从其中释放出来。对在含有¹⁴C-棕榈酸的培养基中多次分裂的培养物提取物进行硅酸柱色谱分析的数据表明，细胞脂质的很大一部分是甘油三酯，但放射性广泛分布于其他脂质中。据估计，在指数后期培养物中，约5%的细胞干重是甘油三酯。
