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豚鼠对化学物质迟发型超敏反应的无细胞被动转移

Cell-free passive transfer of delayed hypersensitivity to chemicals in guinea pigs.

作者信息

Burger D R, Jeter W S

出版信息

Infect Immun. 1971 Nov;4(5):575-80. doi: 10.1128/iai.4.5.575-580.1971.

Abstract

Delayed-type cutaneous reactivity to 2,4-dinitrochlorobenzene and 2,4-dinitrofluorobenzene in guinea pigs was transferred passively by fluids in which leukocytes from sensitive animals were incubated. Cells from peritoneal exudates, lymph nodes, and alveolar washings were employed. The cell-free transfer material was dialyzable, of small molecular size, and stable to 56 C for 30 min and -65 C for at least 9 weeks. It gave a ratio at 280 to 260 nm of 0.71. The relationships between temperature, pH, and cellular release of the transfer material were studied.

摘要

将敏感豚鼠的白细胞置于液体中孵育后,其液体可被动转移对2,4 - 二硝基氯苯和2,4 - 二硝基氟苯的迟发型皮肤反应。使用了来自腹膜渗出液、淋巴结和肺泡灌洗的细胞。无细胞转移物质可透析,分子尺寸小,在56℃下30分钟稳定,在 - 65℃下至少9周稳定。其在280至260nm处的吸光度比值为0.71。研究了温度、pH与转移物质细胞释放之间的关系。

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1
Induction of 'delayed' hypersensitivity.
Nature. 1962 Feb 3;193:492-3. doi: 10.1038/193492a0.

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