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[用于在假单胞菌属分类学中测定作为唯一碳源的物质的亚甲基蓝还原试验(MR试验)和微量TTC试验(作者译)]

[The methylene-blue-reduction-test (MR-test) and the micro-TTC-test for the determination of substances as sole source of carbon in the taxonomy of Pseudomonas species (author's transl)].

作者信息

Schubert R H, Esanu J G, Esanu F

出版信息

Zentralbl Bakteriol Orig B. 1975;160(1):50-9.

PMID:51553
Abstract

Two methods are described for the determination of substances as sole source of carbon for taxonomic tools: the Methylene-Blue-Reduction-Test and the Micro-TTC-Test. Both methods are giving results superior to the technique introduced by STANIER and co-workers and used hitherto in the taxonomic classification of Pseudomonas species. The evaluation of the comparative test results is based on the criterion that a substance is useful for taxonomic purpose provided that at least 90 per cent of the strains of a species are giving homogeneous results. - The MR-Test is superior to the Micro-TTC-Test concerning the reproducibility of the results. The Micro-TTC-Test is useful as a screening technique in the routine work and the primary investigation of numerous strains.

摘要

文中描述了两种将物质作为分类工具唯一碳源的测定方法

亚甲基蓝还原试验和微量TTC试验。这两种方法所得结果均优于斯坦尼尔及其同事所采用且迄今用于假单胞菌属分类的技术。对比试验结果的评估基于这样一个标准,即一种物质若能使某一物种至少90%的菌株得出一致结果,则可用于分类目的。——就结果的可重复性而言,MR试验优于微量TTC试验。微量TTC试验在日常工作及对众多菌株的初步研究中可作为一种筛选技术。

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