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文昌鱼肌肉细胞中的兴奋-收缩偶联

Excitation-contraction coupling in amphioxus muscle cells.

作者信息

Hagiwara S, Henkart M P, Kidokoro Y

出版信息

J Physiol. 1971 Dec;219(1):233-51. doi: 10.1113/jphysiol.1971.sp009659.

DOI:10.1113/jphysiol.1971.sp009659
PMID:5158596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1331627/
Abstract
  1. Excitation-contraction coupling was studied in myotomal muscles of amphioxus, Branchiostoma californiense.2. The action potential of a muscle cell produces a twitch with a rise time of 30-40 msec at 11 degrees C and its Q(10) is about 2.2.3. The twitch increases in amplitude with increasing external Ca concentration and is abolished in Ca-free saline (1 mM-EGTA and 55.7 mM-MgCl(2)); the twitch amplitude is suppressed by Co or La ions.4. Caffeine at concentrations above 1 mM in the external saline causes a prolongation of the action potential and a contracture which lasts several minutes.5. After exposure to caffeine the responsiveness of the muscle to subsequent applications of caffeine recovers in normal saline in 20-30 minutes but not in Ca-free saline.6. The amplitude of the caffeine contracture is independent of the external Ca concentration and is unaltered after the twitch is eliminated in Ca-free saline.7. After exposure to caffeine a full-sized twitch can be obtained before the responsiveness to caffeine shows any significant recovery.8. It is concluded that the twitch is produced by the Ca influx resulting from the increased permeability of the muscle cell membrane to Ca during the action potential and that the Ca mobilized by caffeine is not necessary to the initiation of the twitch.9. Electronmicroscopy shows the existence of sarcoplasmic reticulum.
摘要

  1. 对加州文昌鱼肌节肌的兴奋-收缩偶联进行了研究。

  2. 肌肉细胞的动作电位在11摄氏度时产生一个上升时间为30 - 40毫秒的单收缩，其Q(10)约为2.2。

  3. 单收缩幅度随细胞外钙浓度增加而增大，在无钙盐溶液（1 mM - 乙二醇双四乙酸和55.7 mM - 氯化镁）中消失；单收缩幅度受到钴或镧离子的抑制。

  4. 细胞外盐溶液中咖啡因浓度高于1 mM时会导致动作电位延长和持续数分钟的挛缩。

  5. 暴露于咖啡因后，肌肉对随后施加的咖啡因的反应性在正常盐溶液中20 - 30分钟内恢复，但在无钙盐溶液中则不会恢复。

  6. 咖啡因挛缩的幅度与细胞外钙浓度无关，在无钙盐溶液中消除单收缩后其幅度不变。

  7. 暴露于咖啡因后，在对咖啡因的反应性显示出任何显著恢复之前，可以获得一个全尺寸的单收缩。

  8. 得出的结论是，单收缩是由动作电位期间肌肉细胞膜对钙通透性增加导致的钙内流产生的，并且咖啡因动员的钙对于单收缩的起始不是必需的。

  9. 电子显微镜显示存在肌浆网。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/e1a7a89b072f/jphysiol01007-0258-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/dbaec65abd06/jphysiol01007-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/b54397d0a072/jphysiol01007-0257-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/d1c76bc48d29/jphysiol01007-0257-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/9f2449c4e828/jphysiol01007-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/a859ae1ba520/jphysiol01007-0258-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/6b7915a8d9f1/jphysiol01007-0258-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/e1a7a89b072f/jphysiol01007-0258-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/dbaec65abd06/jphysiol01007-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/b54397d0a072/jphysiol01007-0257-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/d1c76bc48d29/jphysiol01007-0257-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/9f2449c4e828/jphysiol01007-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/a859ae1ba520/jphysiol01007-0258-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/6b7915a8d9f1/jphysiol01007-0258-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8108/1331627/e1a7a89b072f/jphysiol01007-0258-d.jpg

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