Weber A, Herz R
J Gen Physiol. 1968 Nov;52(5):750-9. doi: 10.1085/jgp.52.5.750.
At concentrations between 1 to 10 mM, caffeine reduced the Ca-accumulating capacity of fragmented reticulum obtained from frog and rabbit muscle. With 8 mM caffeine enough Ca was released from frog reticulum to account for the force of the contracture. Caffeine did not affect all reticulum membranes equally. The fraction which was spun down at 2000 g was more sensitive than the lighter fractions. The percentage of the total accumulated Ca released by caffeine decreased with decreasing Ca content of the reticulum. In parallel with their known effects on the caffeine contracture, a drop in temperature increased the caffeine-induced Ca release while procaine inhibited it. Caffeine also inhibited the rate of Ca uptake, which may in part account for the prolongation of the active state caused by caffeine.
在浓度为1至10毫摩尔之间时,咖啡因降低了从青蛙和兔子肌肉中获得的破碎肌质网的钙积累能力。使用8毫摩尔的咖啡因时,青蛙肌质网释放出的钙足以解释挛缩的力量。咖啡因对所有肌质网膜的影响并不相同。在2000克离心力下沉淀的部分比较轻的部分更敏感。随着肌质网钙含量的降低,咖啡因释放的总积累钙的百分比也随之下降。与它们对咖啡因挛缩的已知影响相似,温度下降会增加咖啡因诱导的钙释放,而普鲁卡因则会抑制这种释放。咖啡因还抑制了钙摄取的速率,这可能部分解释了咖啡因导致的活性状态延长。