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乳酸乳球菌I3细胞壁甘油磷壁酸的进一步研究。磷酸二酯基团的位置及其对碱水解的敏感性。

Further studies on the glycerol teichoic acid of walls of Staphylococcus lactis I3. Location of the phosphodiester groups and their susceptibility to hydrolysis with alkali.

作者信息

Archibald A R, Baddiley J, Heckels J E, Heptinstall S

出版信息

Biochem J. 1971 Nov;125(1):353-9. doi: 10.1042/bj1250353.

Abstract
  1. The teichoic acid from walls of Staphylococcus lactis I3 is readily degraded in dilute alkali. 2. Degradation proceeds by selective hydrolysis of that phosphodiester group attached to an alcoholic hydroxyl group of the N-acetylglucosamine and gives a repeating unit in high yield. 3. Further studies on a different repeating unit isolated by partial acid hydrolysis have shown that the glycerol diphosphate is attached to the 4-hydroxyl group of the N-acetylglucosamine and not to the 3-hydroxyl group as was proposed earlier. 4. The susceptibility towards hydrolysis by alkali of other structural types of teichoic acid has been examined and found to vary markedly according to their structure.
摘要
  1. 乳酸链球菌I3细胞壁的磷壁酸在稀碱中很容易降解。2. 降解是通过选择性水解连接在N-乙酰葡糖胺醇羟基上的磷酸二酯基团进行的,并高产率地得到一个重复单元。3. 对通过部分酸水解分离得到的不同重复单元的进一步研究表明,甘油二磷酸连接在N-乙酰葡糖胺的4-羟基上,而不是如先前提出的连接在3-羟基上。4. 已经研究了其他结构类型的磷壁酸对碱水解的敏感性,发现其根据结构的不同有显著差异。

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引用本文的文献

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Immunological properties of teichoic acids.磷壁酸的免疫学特性。
Bacteriol Rev. 1973 Jun;37(2):215-57. doi: 10.1128/br.37.2.215-257.1973.

本文引用的文献

1
Separation of the phosphoric esters on the filter paper chromatogram.磷酸酯在滤纸色谱上的分离
Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0.
3
Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
7
The action of dilute alkali on bacterial cell walls.稀碱对细菌细胞壁的作用。
Biochem J. 1969 Aug;113(5):899-900. doi: 10.1042/bj1130899.

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