Devor K A, Mudd J B
J Lipid Res. 1971 Jul;12(4):412-9.
The acylation of lysophosphatidylcholine by enzyme preparations from spinach leaves was studied. The acylation reaction was followed by the incorporation of (14)C-labeled fatty acids from the respective coenzyme A derivatives into phosphatidylcholine. The subcellular fraction with the highest specific activity was the microsomal fraction. Contaminating thioesterase activity which was encountered was inhibited by treatment with sodium dodecyl sulfate. The acyltransferase activity was only mildly inhibited by sulfhydryl reagents. Labeled fatty acid was primarily incorporated into phosphatidylcholine. When saturated and unsaturated fatty acyl CoA derivatives were used, the saturated derivatives were incorporated primarily into the 1-position of the glycerol moiety, and the unsaturated fatty acids went primarily to the 2-position. This pattern of incorporation agrees with the fatty acid distribution in vivo.
研究了菠菜叶酶制剂对溶血磷脂酰胆碱的酰化作用。酰化反应通过将各自辅酶A衍生物中的（14）C标记脂肪酸掺入磷脂酰胆碱来跟踪。比活性最高的亚细胞部分是微粒体部分。遇到的污染硫酯酶活性通过用十二烷基硫酸钠处理而受到抑制。酰基转移酶活性仅受到巯基试剂的轻微抑制。标记脂肪酸主要掺入磷脂酰胆碱中。当使用饱和和不饱和脂肪酰辅酶A衍生物时，饱和衍生物主要掺入甘油部分的1位，不饱和脂肪酸主要进入2位。这种掺入模式与体内脂肪酸分布一致。
