Singh H, Spritz N, Geyer B
J Lipid Res. 1971 Jul;12(4):473-81.
Myelin was isolated from the brains of "quaking" and littermate control animals and its composition was determined. The brains of quaking animals contained approximately one-fourth as much myelin as the control animals. There were qualitative as well as quantitative differences between the myelin from the two groups. By continuous cesium chloride gradient flotation it was shown that the myelin from the quaking animals consisted solely of a band corresponding to the heavier and smaller of the two bands found in normal controls. Cholesterol and glycolipids were lower and phospholipids (mainly phosphatidylcholine) and protein were higher in quaking animals than in controls. Also, phosphatidal-ethanolamine was decreased, and several consistent differences in the fatty acids (both unsubstituted and hydroxy) and aldehydes of the component lipids were found. In general there were smaller amounts of monounsaturated fatty acids in quaking animals. We suggest from these findings that myelin in the quaking mouse has certain compositional similarities with juvenile myelin, but it may be an abnormal type of myelin.
从“颤抖”小鼠及同窝对照动物的大脑中分离出髓磷脂，并对其成分进行了测定。“颤抖”小鼠大脑中的髓磷脂含量约为对照动物的四分之一。两组髓磷脂在质量和数量上均存在差异。通过连续氯化铯梯度浮选法表明，“颤抖”小鼠的髓磷脂仅由一条带组成，该带对应于正常对照中发现的两条带中较重且较小的那条带。与对照相比，“颤抖”小鼠体内的胆固醇和糖脂含量较低，磷脂（主要是磷脂酰胆碱）和蛋白质含量较高。此外，磷脂酰乙醇胺含量降低，并且在组成脂质的脂肪酸（包括未取代的和羟基化的）和醛类方面发现了几个一致的差异。总体而言，“颤抖”小鼠体内的单不饱和脂肪酸含量较少。基于这些发现，我们认为“颤抖”小鼠的髓磷脂与幼年髓磷脂在某些成分上具有相似性，但它可能是一种异常类型的髓磷脂。
