用光反应性亲脂试剂对鸡蛋磷脂酰胆碱囊泡和髓鞘膜进行标记。
Labelling of egg phosphatidylcholine vesicles and myelin membrane with a photoreactive lipophilic reagent.
作者信息
Abu-Salah K M, Findlay J B
出版信息
Biochem J. 1977 Feb 1;161(2):223-8. doi: 10.1042/bj1610223.
Abstract
The preparation and isolation of [3H]phenyl azide, a photosensitive non-polar probe, is reported. The reagent partitions into the lipid bilayer of egg phosphatidylcholine vesicles and bovine myelin membranes. On photoactivation to generate the nitrene grouping, as much as 90% of the covalently attached label is associated with the fatty acyl residues of the constituent phospholipid molecules. The remainder is found in the polar head groups. The cholesterol component of myelin membranes is also heavily labelled. These results suggest that such reagents may be used to probe the hydrophobic regions of natural membranes.
摘要
报道了一种光敏非极性探针[3H]苯基叠氮的制备与分离。该试剂可分配到卵磷脂囊泡和牛髓鞘膜的脂质双层中。光激活产生氮烯基团后，高达90%的共价连接标记物与组成磷脂分子的脂肪酰基残基相关联。其余部分存在于极性头部基团中。髓鞘膜的胆固醇成分也被大量标记。这些结果表明，此类试剂可用于探测天然膜的疏水区域。
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