含羟脯氨酸残基的原胶原模型肽的合成与酶促羟化作用
Synthesis and enzymic hydroxylation of protocollagen model peptide containing a hydroxyproline residue.
作者信息
Kikuchi Y, Fujimoto D, Tamiya N
出版信息
Biochem J. 1971 Oct;124(4):695-700. doi: 10.1042/bj1240695.
Abstract
- Two protocollagen model peptides, Z-Gly-Pro-Hyp-Gly-(Pro-Pro-Gly)(5) (Z, benzyloxycarbonyl) and AOC-(Pro-Pro-Gly)(6) (AOC, tert.-pentyloxycarbonyl), were synthesized and hydroxylated with protocollagen proline hydroxylase. 2. The two model peptides were hydroxylated equally. The results suggest that the hydroxyl group of hydroxyproline contained in the N-terminal region of the peptide has no effect on the enzymic hydroxylation of the model peptide.
摘要
- 合成了两种原胶原模型肽，Z-甘氨酰-脯氨酰-羟脯氨酰-甘氨酰-(脯氨酰-脯氨酰-甘氨酰)5（Z，苄氧羰基）和AOC-(脯氨酰-脯氨酰-甘氨酰)6（AOC，叔戊氧羰基），并用原胶原脯氨酸羟化酶进行羟化。2. 这两种模型肽被同等程度地羟化。结果表明，肽N端区域所含羟脯氨酸的羟基对模型肽的酶促羟化没有影响。
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Synthesis and enzymic hydroxylation of protocollagen model peptide containing a hydroxyproline residue.含羟脯氨酸残基的原胶原模型肽的合成与酶促羟化作用
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