原胶原模型的酶促羟基化作用。
The enzymic hydroxylation of protocollagen models.
作者信息
Kikuchi Y, Fujimoto D, Tamiya N
出版信息
Biochem J. 1969 Nov;115(3):569-74. doi: 10.1042/bj1150569.
Abstract
- Synthetic polymers of l-prolyl-l-prolylglycine of defined chain length, (Pro-Pro-Gly)(n), were found to be substrates for the enzyme protocollagen-proline hydroxylase, with optimum chain length n=5. Boiling the polymer (Pro-Pro-Gly)(15) increased its activity as a substrate but had no effect on (Pro-Pro-Gly)(5). 2. Protection of both or one of the N- and C-terminal groups made (Pro-Pro-Gly)(3) a better substrate, and collagenase digestion of hydroxylated tert.-pentyloxy-carbonyl-(Pro-Pro-Gly)(3) benzyl ester indicated that the central prolyl residues were the major points of hydroxylation. 3. The results suggest that the long-chain peptides are optimum substrates but that a triple-stranded structure is inhibitory for hydroxylation.
摘要
- 已发现具有特定链长的L-脯氨酰-L-脯氨酰甘氨酸的合成聚合物（Pro-Pro-Gly）(n)是原胶原-脯氨酸羟化酶的底物，最佳链长为n = 5。将聚合物（Pro-Pro-Gly）(15)煮沸可提高其作为底物的活性，但对（Pro-Pro-Gly）(5)没有影响。2. 对N端和C端基团中的一个或两个进行保护，使（Pro-Pro-Gly）(3)成为更好的底物，对羟基化的叔戊氧基羰基-（Pro-Pro-Gly）(3)苄酯进行胶原酶消化表明，中间的脯氨酰残基是主要的羟基化位点。3. 结果表明，长链肽是最佳底物，但三链结构对羟基化具有抑制作用。
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3
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Adv Protein Chem. 1961;16:1-138. doi: 10.1016/s0065-3233(08)60028-5.
4
Hydroxylation of poly(l-prolyl-l-prolylglycyl) of defined molecular weights by protocollagen proline hydroxylase.原胶原蛋白脯氨酸羟化酶对特定分子量的聚（L-脯氨酰-L-脯氨酰甘氨酰）的羟化作用。
FEBS Lett. 1969 Feb;2(4):221-223. doi: 10.1016/0014-5793(69)80024-4.
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