Bowen I D, Ryder T A, Winters C
Cell Tissue Res. 1975 Aug 18;161(2):263-75. doi: 10.1007/BF00220373.
A chromic acid oxidation-silver technique was used to localize polysaccharide material in Polycelis tenuis at the electron microscope level. In the epithelium, staining was observed within apical vacuoles and on the free surfaces of the cells. A similar staining was observed in relation to the glycocalyx of the pharyngeal epithelia and that of the flame cells. Silver was deposited in the basement membrane. In the parenchyma, the major components giving a positive reaction were the cyanophil and mucous gland cells. Particularly strong silver staining (confirmed by X-ray microanalysis) was observed in the granules and Golgi apparatus of the cyanophil cells. IDPase activity was also found in relation to the Golgi apparatus and its secretory products. The overall distribution of mucopolysaccharide material was confirmed with the PAS and Alcian blue techniques. The fine structural localization of the Alcian blue was also determined using electron microscopy and X-ray microanalysis.
采用铬酸氧化-银技术在电子显微镜水平定位细颈涡虫体内的多糖物质。在上皮组织中，可观察到顶端液泡内及细胞游离表面有染色现象。在咽上皮和焰细胞的糖萼处也观察到类似染色。银沉积于基底膜。在实质组织中，呈阳性反应的主要成分是嗜蓝细胞和黏液腺细胞。在嗜蓝细胞的颗粒和高尔基体中观察到特别强烈的银染色（经X射线微分析证实）。在高尔基体及其分泌产物中也发现了IDPase活性。用PAS和阿尔辛蓝技术证实了黏多糖物质的整体分布。还利用电子显微镜和X射线微分析确定了阿尔辛蓝的精细结构定位。
