Rudel M, Gabert A
Acta Biol Med Ger. 1979;38(1):41-5.
A procedure for preparing derivatives of dialdehyde dextran-glass-carriers and their use for immobilization of trypsin is described. The dextran to the support as well as the trypsin to dextran were covalently immobilized via dialdehyde groups. The relative activity of immobilized trypsin in these enzyme-carrier-complexes amounted 17.3% and 20,4 (determined with alpha-N-Benzoyl-DL-arginine-p-nitroanilid) and 8,2% and 11.6% (determined with casein).
本文描述了一种制备二醛葡聚糖-玻璃载体衍生物的方法及其用于固定化胰蛋白酶的用途。葡聚糖与载体以及胰蛋白酶与葡聚糖通过二醛基团共价固定。这些酶-载体复合物中固定化胰蛋白酶的相对活性分别为17.3%和20.4%（用α-N-苯甲酰-DL-精氨酸对硝基苯胺测定）以及8.2%和11.6%（用酪蛋白测定）。
