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线粒体甾体羟化系统的分离、结构组织及作用机制

Isolation, structural organization and mechanism of action of mitochondrial steroid hydroxylating systems.

作者信息

Akhrem A A, Lapko V N, Lapko A G, Shkumatov V M, Chashchin V L

出版信息

Acta Biol Med Ger. 1979;38(2-3):257-73.

PMID:517002
Abstract

A scheme for the isolation of individual proteins of the 20S,22R-cholesterol hydroxylating system is presented. Physico-chemical and structural parameters of cytochrome P-450 are furnished. Self-reconstitution of indivudal proteins of the system into an enzyme unit is shown. Furthermore a regulation process of electron transport in the 20S,22R-cholesterol system is considered.

摘要

本文提出了一种分离20S,22R-胆固醇羟化系统中单个蛋白质的方案。提供了细胞色素P-450的物理化学和结构参数。展示了该系统中单个蛋白质自我重组为酶单位的过程。此外，还考虑了20S,22R-胆固醇系统中电子传递的调节过程。

相似文献

1
Isolation, structural organization and mechanism of action of mitochondrial steroid hydroxylating systems.线粒体甾体羟化系统的分离、结构组织及作用机制
Acta Biol Med Ger. 1979;38(2-3):257-73.
2
[Mechanism of electron transport in the cholesterol-hydroxylating system of adrenal cortex mitochondria: a triple complex of adrenodoxin reductase, adrenodoxin and cytochrome P-450].[肾上腺皮质线粒体胆固醇羟化系统中的电子传递机制：肾上腺皮质铁氧还蛋白还原酶、肾上腺皮质铁氧还蛋白和细胞色素P-450的三联体复合物]
Biokhimiia. 1988 Aug;53(8):1352-6.
3
[Comparative study of the C27-steroid-hydroxylating system from bovine liver mitochondria].[牛肝线粒体C27-类固醇羟化系统的比较研究]
Biokhimiia. 1987 Feb;52(2):198-213.
4
Purification of adrenodoxin reductase, adrenodoxin, and cytochrome P-450 from adrenal cortex.
Methods Enzymol. 1978;52:132-42. doi: 10.1016/s0076-6879(78)52015-6.
5
[Regulation of electron transport in the 20S, 22R-cholesterol hydroxylating system of adrenal cortex mitochondria].[肾上腺皮质线粒体20S、22R-胆固醇羟化系统中电子传递的调节]
Dokl Akad Nauk SSSR. 1979;245(6):1490-3.
6
[Localization of the adrenodoxin-binding fragment of cholesterol hydroxylating cytochrome P-450 from adrenal cortex mitochondria].[肾上腺皮质线粒体胆固醇羟化细胞色素P-450的肾上腺皮质铁氧还蛋白结合片段的定位]
Bioorg Khim. 1985 Jan;11(1):135-7.
7
Purification of adrenodoxin reductase from bovine adrenal cortex mitochondria by affinity chromatography. Properties of steroid hydroxylase systems reconstituted from adrenodoxin reductase, adrenodoxin and membranous cytochrome P-450(11)beta.通过亲和色谱法从牛肾上腺皮质线粒体中纯化肾上腺皮质铁氧还蛋白还原酶。由肾上腺皮质铁氧还蛋白还原酶、肾上腺皮质铁氧还蛋白和膜结合细胞色素P-450(11)β重构的类固醇羟化酶系统的性质。
J Steroid Biochem. 1979 Nov-Dec;11(5-6):1551-9. doi: 10.1016/0022-4731(79)90348-0.
8
[11-beta-Steroid hydroxylating cytochrome P-450 from bovine adrenal cortex mitochondria: isolation, physico-chemical and functional properties].[来自牛肾上腺皮质线粒体的11-β-类固醇羟化细胞色素P-450：分离、物理化学及功能特性]
Biokhimiia. 1982 Jul;47(7):1070-83.
9
[Molecular organization of the reductase complex in adrenal cortex mitochondria. Study using bifunctional reagents].[肾上腺皮质线粒体中还原酶复合物的分子组织。使用双功能试剂的研究]
Bioorg Khim. 1986 Feb;12(2):185-94.
10
[Reconstruction and study of a multi-enzyme system by 11 beta-hydroxylase steroids].[11β-羟化酶类固醇对多酶系统的重建与研究]
Biokhimiia. 1985 Feb;50(2):243-57.

引用本文的文献

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Analysis of In Vivo Activity of the Bovine Cholesterol Hydroxylase/Lyase System Proteins Expressed in Escherichia coli.对在大肠杆菌中表达的牛胆固醇羟化酶/裂解酶系统蛋白质的体内活性分析。
Mol Biotechnol. 2019 Apr;61(4):261-273. doi: 10.1007/s12033-019-00158-6.
2
Expression of Cholesterol Hydroxylase/Lyase System Proteins in Yeast S. cerevisiae Cells as a Self-Processing Polyprotein.胆固醇羟化酶/裂解酶系统蛋白在酿酒酵母细胞中作为一种自我加工多聚蛋白的表达。
Mol Biotechnol. 2017 Oct;59(9-10):394-406. doi: 10.1007/s12033-017-0028-5.
3
Atomic Force Microscopy Study of Protein-Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System.
含细胞色素CYP11A1（P450scc）的类固醇羟化酶系统中蛋白质-蛋白质相互作用的原子力显微镜研究。
Nanoscale Res Lett. 2011 Dec;6(1):54. doi: 10.1007/s11671-010-9809-5. Epub 2010 Sep 30.
4
Protein phosphorylation and intermolecular electron transfer: a joint experimental and computational study of a hormone biosynthesis pathway.蛋白质磷酸化与分子间电子转移：激素生物合成途径的联合实验与计算研究
J Am Chem Soc. 2007 Apr 11;129(14):4206-16. doi: 10.1021/ja064803j. Epub 2007 Mar 15.
5
A pathway for the metabolism of vitamin D3: unique hydroxylated metabolites formed during catalysis with cytochrome P450scc (CYP11A1).维生素D3的代谢途径：在细胞色素P450scc（CYP11A1）催化过程中形成的独特羟基化代谢产物。
Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14754-9. doi: 10.1073/pnas.2336107100. Epub 2003 Dec 1.