Bray B A, Hsu K C, Wigger H J, LeRoy E C
Connect Tissue Res. 1975;3(1):55-71. doi: 10.3109/03008207509152342.
Two non-collagenous placental antigens have been detected both in membranes isolated from terminal villi by sieving and sonication and in acetic acid extracts of the villi. One of the antigens is apparently fibrinogen based on immunologic and chemical data. The second, detectable with anti-membrane antiserum after absorption with fibrinogen remained with the fibrinogen-like antigen during isoelectric precipitation at pH 4.7, electrophoresis at pH 8.6 and chromatography on DEAE cellulose. Fractions of villi in which the two antigens occur comprise more than a third of the weight of the villi. The fibrinogen-related antigen was concentrated in placental basement membranes compared to kidney and lung basement membranes. A third antigen common to the membranes, to glomerular basement membrane and to alveolar basement membrane was also detected.
通过筛分和超声处理从终末绒毛分离的膜以及绒毛的醋酸提取物中，已检测到两种非胶原蛋白胎盘抗原。根据免疫学和化学数据，其中一种抗原显然是纤维蛋白原。第二种抗原在用纤维蛋白原吸收后的抗膜抗血清中可检测到，在pH 4.7的等电沉淀、pH 8.6的电泳以及DEAE纤维素色谱分析过程中，它与纤维蛋白原样抗原一起存在。含有这两种抗原的绒毛部分占绒毛重量的三分之一以上。与肾和肺基底膜相比，纤维蛋白原相关抗原在胎盘基底膜中富集。还检测到一种存在于胎盘膜、肾小球基底膜和肺泡基底膜中的第三种共同抗原。
