Wieslander J, Bygren P, Heinegård D
Biochim Biophys Acta. 1979 May 17;553(2):244-54. doi: 10.1016/0005-2736(79)90229-3.
Human glomerular basement membrane was solubilized by digestion with proteolytic enzymes and immunoreactive components were quantitated and characterized by using rabbit antibodies raised against the particulate membrane. A number of antigens were demonstrated but they did not separate on gel filtration. However, two antigenic components in a collagenase digest of the membrane could be separated and isolated by Sepharose 6B chromatography. Chemical characterization suggests that both fragments are noncollagenous glycopeptides (molecular weights approx. 1,000,000 and 60,000--200,000, respectively).
用人肾小球基底膜经蛋白水解酶消化使其溶解，并用针对颗粒膜制备的兔抗体对免疫反应性成分进行定量和特性鉴定。证实了多种抗原，但它们在凝胶过滤时不能分离。然而，该膜胶原酶消化物中的两种抗原成分可通过琼脂糖6B层析分离和纯化。化学特性表明，这两种片段均为非胶原糖肽（分子量分别约为1,000,000和60,000 - 200,000）。
