Kurchenko V P, Usanov S A, Metelintsa D I
Biokhimiia. 1982 Sep;47(9):1431-6.
A systematic study of inhibition by antibodies of dimethylaniline (DMA) and aniline oxidation has been carried out under different conditions: e. g. with participation of intact, phenobarbital- and 3-methylcholantrene-induced microsomes, NADPH and O2; during hydroperoxide-dependent oxidation with three types of microsomes; in a reconstituted system containing cytochrome P-450 LM2, NADPH cytochrome P-450 reductase, NADPH and O2; in systems containing cytochrome P-450 LM2 and cumene hydroperoxide. In all cases the antibodies effectively inhibited oxidation of both substrates. The degree of inhibition increased in the following order: intact less than 3-methylcholantrene- less than phenobarbital-induced microsomes. In the case of hydroperoxide-dependent oxidation of aniline and DMA no complete inhibition was achieved.
在不同条件下对抗体抑制二甲基苯胺（DMA）和苯胺氧化进行了系统研究：例如，在完整的、经苯巴比妥和3-甲基胆蒽诱导的微粒体、NADPH和O₂参与的情况下；在用三种类型微粒体进行的氢过氧化物依赖性氧化过程中；在含有细胞色素P-450 LM2、NADPH细胞色素P-450还原酶、NADPH和O₂的重组系统中；在含有细胞色素P-450 LM2和异丙苯氢过氧化物的系统中。在所有情况下，抗体均有效抑制了两种底物的氧化。抑制程度按以下顺序增加：完整微粒体＜3-甲基胆蒽诱导的微粒体＜苯巴比妥诱导的微粒体。在苯胺和DMA的氢过氧化物依赖性氧化情况下，未实现完全抑制。
