Behrens J A, Brown W P, Gibson D F, Detter J C
Am J Clin Pathol. 1979 Dec;72(6):904-8. doi: 10.1093/ajcp/72.6.904.
The Coulter Model S provides an accurate measurement of total hemoglobin (Hb) content in peripheral blood even when elevated levels of carboxyhemoglobin (HbCO) are present. Commercial standards and diluents for spectrophotometric determinations of cyanmethemoglobin (HiCN) are variable in quality. All diluents studied produce turbidity in some samples, and this may explain why manual Hb determinations tend to be higher than hemoglobin measurement on the Coulter S. The most reliable diluent was the reagent described by Van Kampen and Zijlstra. Its reliability is enhanced by centrifugation of sample in diluent. The use of a narrow-band-pass spectrophotometer for routine HiCN determinations in a clinical laboratory is not recommended because of its sensitivity to turbidity and HbCO.
库尔特S型血细胞分析仪即使在存在高浓度碳氧血红蛋白（HbCO）的情况下，也能准确测量外周血中的总血红蛋白（Hb）含量。用于分光光度法测定氰化高铁血红蛋白（HiCN）的商业标准品和稀释剂质量参差不齐。所研究的所有稀释剂在某些样本中都会产生浑浊，这可能解释了为什么手工测定的Hb值往往高于库尔特S型血细胞分析仪测量的血红蛋白值。最可靠的稀释剂是范坎彭和齐尔斯特拉描述的试剂。通过在稀释剂中对样本进行离心可提高其可靠性。不建议在临床实验室中使用窄带通分光光度计进行常规HiCN测定，因为它对浑浊和HbCO敏感。
