Rossi-Bernardi L, Perella M, Luzzana M, Samaja M, Raffaele I
Clin Chem. 1977 Jul;23(7):1215-25.
We describe a new method for simultaneous determination of four hemoglobin derivatives (deoxyhemoglobin, Hb; oxyhemoglobin, HbO2; methemoglobin, Hb+; and carbon monoxide hemoglobin, HbCO) and total oxygen content in 10 microliters of whole blood. Percentage HbO2, HbCO, Hb+, total hemoglobin (Hbt), and oxygen capacity can also be obtained from the experimental data by simple calculations. Total analysis time is 1 min. Blood is diluted 100-fold with a buffer contained in a quasi-anaerobic cuvette, where simultaneous measurements of oxygen pressure (by a po2 electrode) and adsorbance (at 497, 565, and 620 nm) are made. The decrease of oxygen pressure, as recorded by the oxygen electrode, is proportional to the amount of deoxyhemoglobin. The concentrations of HbO2, HbCO, and Hb+ can be obtained from absorbance measurements at the specified wavelengths. The new method eliminates the use of short-path optical cells and, due to the low sample volume requirement, makes possible the automated measurement of hemoglobin derivatives and oxygen saturation in arterialized capillary blood.
我们描述了一种新方法，可同时测定10微升全血中的四种血红蛋白衍生物（脱氧血红蛋白，Hb；氧合血红蛋白，HbO₂；高铁血红蛋白，Hb⁺；和一氧化碳血红蛋白，HbCO）以及总氧含量。通过简单计算，还可从实验数据中获得HbO₂、HbCO、Hb⁺、总血红蛋白（Hbt）的百分比以及氧容量。总分析时间为1分钟。血液用准厌氧比色皿中所含的缓冲液稀释100倍，在此同时测量氧分压（通过po₂电极）和吸光度（在497、565和620nm处）。氧电极记录的氧分压下降与脱氧血红蛋白的量成正比。HbO₂、HbCO和Hb⁺的浓度可从指定波长处的吸光度测量值中获得。该新方法无需使用短程光学池，并且由于所需样品体积小，使得动脉化毛细血管血中血红蛋白衍生物和氧饱和度的自动测量成为可能。
