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免疫电泳法在培养肝细胞血浆蛋白合成研究中的应用。

Application of electroimmunoassay to the study of plasma protein synthesis in cultured hepatocytes.

作者信息

Grieninger G, Pindyck J, Hertzberg K M, Mosesson M W

出版信息

Ann Clin Lab Sci. 1979 Nov-Dec;9(6):511-7.

PMID:518014
Abstract

Electroimmunoassay has been applied to the study of plasma protein synthesis and secretion in liver cell cultures. The assay is performed on unconcentrated samples of culture medium containing the secreted plasma proteins and yields results within 2 hours. The characteristics of plasma protein production by the cultured hepatocytes coupled with the sensitivity of this assay permit the study of plasma protein in synthesis and its regulation by hormones and other agents without the routine use of radioisotopes.

摘要

免疫电泳分析法已应用于肝细胞培养中血浆蛋白合成与分泌的研究。该分析是在含有分泌型血浆蛋白的未浓缩培养基样品上进行的，2小时内即可得出结果。培养的肝细胞产生血浆蛋白的特性以及该分析方法的灵敏度，使得在不常规使用放射性同位素的情况下，研究血浆蛋白的合成及其受激素和其他因子的调节成为可能。

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Application of electroimmunoassay to the study of plasma protein synthesis in cultured hepatocytes.免疫电泳法在培养肝细胞血浆蛋白合成研究中的应用。
Ann Clin Lab Sci. 1979 Nov-Dec;9(6):511-7.
2
Synthesis and secretion of plasma proteins by embryonic chick hepatocytes: changing patterns during the first three days of culture.胚胎期鸡肝细胞合成与分泌血浆蛋白：培养头三天的变化模式
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Biosynthesis of plasma proteins in serum-free medium by primary monolayer culture of rat hepatocytes.大鼠肝细胞原代单层培养在无血清培养基中血浆蛋白的生物合成。
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The transport of hemin and protoporphyrin across the plasma membrane of chick embryo liver cells in culture.血红素和原卟啉在培养的鸡胚肝细胞质膜上的转运。
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Some observations on the protein synthesis in cultivated chicken embryonic liver.关于培养的鸡胚肝脏中蛋白质合成的一些观察
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Snythesis and differentiation of plasma proteins in cultured embryonic chicken liver cells: a system for study of regulation of protein synthesis.培养的鸡胚肝细胞中血浆蛋白的合成与分化：一种研究蛋白质合成调控的系统
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Effects of nutritional and hormonal factors on the metabolism of retinol-binding protein by primary cultures of rat hepatocytes.营养和激素因素对大鼠肝细胞原代培养物中视黄醇结合蛋白代谢的影响。
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Immunofluorescence localization of plasma protein synthesis in cultured chick hepatocytes.培养的鸡肝细胞中血浆蛋白合成的免疫荧光定位
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Chronic iron overload inhibits protein secretion by adult rat hepatocytes maintained in long-term primary culture.慢性铁过载会抑制长期原代培养的成年大鼠肝细胞的蛋白质分泌。
Eur J Cell Biol. 1989 Jun;49(1):162-70.

引用本文的文献

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Direct effect of insulin on the synthesis of specific plasma proteins: biphasic response of hepatocytes cultured in serum- and hormone-free medium.胰岛素对特定血浆蛋白合成的直接作用：在无血清和无激素培养基中培养的肝细胞的双相反应。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6972-6. doi: 10.1073/pnas.78.11.6972.
2
Hereditary tyrosinemia and the heme biosynthetic pathway. Profound inhibition of delta-aminolevulinic acid dehydratase activity by succinylacetone.遗传性酪氨酸血症与血红素生物合成途径。琥珀酰丙酮对δ-氨基乙酰丙酸脱水酶活性的深度抑制。
J Clin Invest. 1983 Mar;71(3):625-34. doi: 10.1172/jci110809.
3
Effects of dimethyl sulphoxide on the synthesis of plasma proteins in the human hepatoma HepG2. Induction of an acute-phase-like reaction.
二甲基亚砜对人肝癌HepG2细胞血浆蛋白合成的影响。诱导急性期样反应。
Biochem J. 1988 Aug 1;253(3):927-30. doi: 10.1042/bj2530927.
4
Effect of hepatocyte-stimulating factor and glucocorticoids on plasma fibronectin levels.肝细胞刺激因子和糖皮质激素对血浆纤连蛋白水平的影响。
Biochem J. 1986 Sep 1;238(2):365-71. doi: 10.1042/bj2380365.
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Induction of reversible shock by Escherichia coli lipopolysaccharide in rats. Changes in serum and cell membrane parameters.大肠杆菌脂多糖诱导大鼠可逆性休克。血清和细胞膜参数的变化。
Br J Exp Pathol. 1988 Dec;69(6):805-12.
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The effects of acute-phase inducers and dimethyl sulphoxide on delta-aminolaevulinate synthase activity in human HepG2 hepatoma cells.急性期诱导剂和二甲基亚砜对人HepG2肝癌细胞中δ-氨基乙酰丙酸合酶活性的影响。
Biochem J. 1989 Apr 15;259(2):605-7. doi: 10.1042/bj2590605.