Pitts O M, Varitek V A, Day E D
J Immunol. 1975 Oct;115(4):1114-6.
A time-course study was made of the systemic humoral immune response of Lewis rats to myelin basic protein (BP) as influenced by the dosage of ancillary pertussis adjuvant. Peak activities were observed 5 to 7 weeks after injection. When injected proximal to BP and Mycobacterium butyricum in complete Freund's adjuvant (CFA), Bordetella pertussis at the level of 4 billion organisms doubled the antibody-binding activity of rat sera for 125I-labeled BP as compared to activities obtained with 0, 2, 6, or 8 billion. The severity of clinical symptoms of experimental allergic encephalomyelitis (EAE) at the end of the 2nd week was greatest in rats receiving 64 billion organisms, the very same rats that displayed a severely dampened humoral immune response to BP 5 weeks later. When pertussis was injected i.p. rather than proximal to the CFA mixture, the time-course of the humoral immune response displayed a different profile--unusually high binding activities at the time of onset of EAE that fluctuated back and forth from high to low and that eventually dampened to an intermediate level.
研究了辅助百日咳佐剂剂量对Lewis大鼠针对髓鞘碱性蛋白（BP）的全身体液免疫反应的时间进程影响。注射后5至7周观察到峰值活性。当在完全弗氏佐剂（CFA）中与BP和丁酸分枝杆菌在近端一起注射时，40亿个生物体水平的百日咳博德特氏菌使大鼠血清对125I标记的BP的抗体结合活性比使用0、2、6或80亿个生物体时获得的活性增加了一倍。在第2周结束时，实验性变应性脑脊髓炎（EAE）临床症状的严重程度在接受640亿个生物体的大鼠中最大，而正是这些大鼠在5周后对BP表现出严重减弱的体液免疫反应。当百日咳通过腹腔注射而不是在CFA混合物近端注射时，体液免疫反应的时间进程呈现出不同的特征——在EAE发作时具有异常高的结合活性，从高到低来回波动，最终减弱到中间水平。
