Watanabe K, Hakomori S
Biochemistry. 1979 Nov 27;18(24):5502-4. doi: 10.1021/bi00591a037.
A novel ganglioside having both N-acetylgalactosamine and N-acetylglucosamine was isolated from human erythrocyte membranes. Its structure was characterized by successive exoglycosidase treatment, methylation analysis, and direct-probe mass spectrometry of permethylated intact and desialylated glycolipid. The core structure of the ganglioside was found to be N-acetylgalactosaminyl paragloboside with sialosyl substitution by 2 leads to 3 linkage at the terminal GalNAc residue: NeuNAc alpha 2 leads to 3 GalNAc beta 1 leads to -3Gal beta 1 leads to 4 GlcNAc beta 1 leads to 3 Gal beta 1 leads to 4Glc leads to Cer. The ganglioside was characterized by the presence of a ceramide with myristic acid as the major component.
从人红细胞膜中分离出一种同时含有N-乙酰半乳糖胺和N-乙酰葡糖胺的新型神经节苷脂。通过连续的外切糖苷酶处理、甲基化分析以及对全甲基化完整和去唾液酸化糖脂的直接探针质谱分析对其结构进行了表征。发现该神经节苷脂的核心结构为N-乙酰半乳糖胺基副球蛋白，在末端GalNAc残基上通过2至3连接进行唾液酸基取代：NeuNAcα2至3GalNAcβ1至3Galβ1至4GlcNAcβ1至3Galβ1至4Glc至Cer。该神经节苷脂的特征是存在以肉豆蔻酸为主要成分的神经酰胺。
