Alison M R, Wright N A
Cell Tissue Kinet. 1979 Sep;12(5):461-75. doi: 10.1111/j.1365-2184.1979.tb00169.x.
The proliferative response to testosterone in the accessory sex glands (seminal vesicle and coagulating gland) of castrated pale Balb/c mice has been examined by pulse and continuous thymidine-labelling experiments, and by the fraction of labelled mitoses technique. Progressive reductions in cellularity followed castration, and by varying the time elapsing between castration and the initiation of testosterone treatment, it was clear that the size of the response depended upon the number of cells in the tissue, relative to the normal complement. Interpretation of FLM data was difficult in periods where proliferative rates changed rapidly. We have attempted to explain the cell kinetic events by postulating a G0 compartment, form which cells are stimulated to enter the proliferative cycle before subsequently returning to an out of cycle state. It was thought unlikely that substantial changes in cell cycle time occurred. In both the accessory sex glands, the overall form of the continuous thymidine labelling curves showed that most proliferative cells entered DNA synthesis in a shorter time after stimulation at 14 days after castration than they did at 3 days after castration. The data were not consistent with cells moving deeper into G0 with time after castration. In the seminal vesicle almost all epithelial cells were potentially proliferative by 3 days after castration. In the coagulating gland only 30% were potentially proliferative at 3 days, increasing to 85% at 14 days after castration. However, such proportional increases represented much smaller changes in terms of absolute numbers of cells, because of a concomitant decline in cellularity from 3 to 14 days after castration.
通过脉冲和连续胸腺嘧啶核苷标记实验以及标记有丝分裂分数技术，研究了去势的浅色Balb/c小鼠附属性腺（精囊和凝固腺）对睾酮的增殖反应。去势后细胞数量逐渐减少，通过改变去势与开始睾酮治疗之间的时间间隔，很明显反应的大小取决于组织中的细胞数量相对于正常细胞数量的比例。在增殖率快速变化的时期，对标记有丝分裂分数（FLM）数据的解释很困难。我们试图通过假设一个G0区室来解释细胞动力学事件，细胞从这个区室被刺激进入增殖周期，随后再回到非周期状态。人们认为细胞周期时间不太可能发生实质性变化。在两个附属性腺中，连续胸腺嘧啶核苷标记曲线的总体形式表明，与去势后3天相比，去势后14天刺激后，大多数增殖细胞在更短时间内进入DNA合成。这些数据与去势后细胞随着时间推移更深地进入G0状态不一致。在精囊中，去势后3天几乎所有上皮细胞都有增殖潜力。在凝固腺中，去势后3天只有30%有增殖潜力，去势后14天增加到85%。然而，由于去势后3至14天细胞数量同时下降，这种比例增加在细胞绝对数量方面代表的变化要小得多。
