Alison M R, Wright N A, Morley A R, Appleton D R
J Microsc. 1976 Mar;106(2):221-37. doi: 10.1111/j.1365-2818.1976.tb02403.x.
Cell proliferation during 100 h of continuous androgen challenge was studied in the seminal vesicle and coagulating gland of Balb/c mice castrated 3 days or 14 days prior to the first daily injection of 250 mug testosterone propionate. Continuous labelling with [3H] thymidine indicated that the seminal vesicle was almost totally responsive to androgen, as early as 3 days after castration, whereas the androgen sensitivity of the coagulating gland increased from 30% at 3 days after castration to 85% at 14 days after castration. In both tissues the magnitude of the proliferative reaction could be related to the extent of cell loss prior to stimulation. The duration of the pre-replicative phase in the response of the seminal vesicle to androgen was 20-25 h both at 3 and 14 days after castration. In the coagulating gland the pre-replicative phase was 40 h at 3 days after castration and 20 h at 14 days after castration. The maximum uptake of [7alpha-3H] testosterone administered to mice 3 days after castration was significantly greater (P less than 0-01) in the seminal vesicle compared to the coagulating gland. At 14 days the seminal vesicle and coagulating gland exhibited a similar capacity for uptake. The in vivo metabolism of [7alpha-3H] testosterone was studied by thin layer chromatography 30 min and 120 min after administration. A high proportion of the radioactivity extracted from all the tissues was associated with highly polar steroids. At 3 days after castration, the seminal vesicle, 2 h after administration of radioactive testosterone, retained a much higher proportion of radioactivity associated with dihydrotestosterone than did the coagulating gland. The localization of steroid in mice 3 days after castration was studied by dry-mount autoradiography at intervals up to 2 h after the injection of [1,2,6,7(n)-3H]-testosterone. A heavier deposition of silver grains was observed over autoradiographs of the seminal vesicle. In the seminal vesicle the grains were primarily located over nuclear areas whereas in the coagulating gland the grains were diffusely distributed over both nuclear areas and over cytoplasmic areas.
在首次每日注射250微克丙酸睾酮前3天或14天对Balb/c小鼠进行去势，研究连续雄激素刺激100小时期间精囊和凝固腺中的细胞增殖。用[3H]胸腺嘧啶连续标记表明，早在去势后3天，精囊对雄激素几乎完全有反应，而凝固腺的雄激素敏感性从去势后3天的30%增加到去势后14天的85%。在这两种组织中，增殖反应的程度可能与刺激前细胞损失的程度有关。精囊对雄激素反应中复制前期的持续时间在去势后3天和14天均为20 - 25小时。在凝固腺中，复制前期在去势后3天为40小时，在去势后14天为20小时。去势后3天给小鼠注射的[7α - 3H]睾酮，其在精囊中的最大摄取量显著高于凝固腺（P小于0.01）。在14天时，精囊和凝固腺表现出相似的摄取能力。给药后30分钟和120分钟，通过薄层色谱法研究了[7α - 3H]睾酮的体内代谢。从所有组织中提取的放射性物质中，很大一部分与高极性类固醇相关。去势后3天，在注射放射性睾酮2小时后，精囊中与双氢睾酮相关的放射性保留比例远高于凝固腺。在注射[1,2,6,7(n) - 3H] - 睾酮后，通过干板放射自显影术在长达2小时的间隔内研究去势后3天小鼠体内类固醇的定位。在精囊的放射自显影片上观察到银粒沉积更重。在精囊中，银粒主要位于核区域，而在凝固腺中，银粒在核区域和细胞质区域均呈弥散分布。
