一种用于测定碱性磷酸酶同工酶血清活性的改良失活抑制法。
A modified inactivation-inhibition method for determining the serum activity of alkaline phosphatase isoenzymes.
作者信息
Stĕpán J, Volek V, Kolár J
出版信息
Clin Chim Acta. 1976 May 17;69(1):1-9. doi: 10.1016/0009-8981(76)90465-4.
PMID:5210
Abstract
A procedure using heat inactivation and L-phenylalanine inhibition to quantitate the activities of bone, liver and intestinal alkaline phosphatase isoenzymes in human serum was confirmed by alkaline phosphatase isoenzyme analysis using an electrophoretic procedure. The results of this assay were compared with the radionuclear 85Sr test, and gamma-glutamyl transpeptidase activity in a group of patients with hepatobiliary and bone diseases.
摘要
采用热灭活和L-苯丙氨酸抑制法对人血清中骨、肝和肠碱性磷酸酶同工酶活性进行定量的方法，通过电泳法进行碱性磷酸酶同工酶分析得到了证实。将该检测结果与放射性核素85Sr试验以及一组肝胆疾病和骨疾病患者的γ-谷氨酰转肽酶活性进行了比较。
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