Gusterson B A, Monaghan P
Invest Cell Pathol. 1979 Jul-Sep;2(3):171-9.
The process of differentiation in keratinocytes of human buccal mucosa is accompanied by a number of specific membrane and cytoplasmic changes. Using simple tissue culture techniques, it has been possible to establish an in vitro model for keratinocyte differentiation which closely resembles the in vivo situation. A multi-layered structure is formed with maturation towards the surface. The superficial cells are characterized by a thickening of the plasma membrane, an increased concentration of tonofilaments, loss of intercellular adhesions and the presence of membrane-coated granules. It is concluded that this represents a suitable in vitro model for the biological and pathological study of both normal and abnormal oral epithelium.
人颊黏膜角质形成细胞的分化过程伴随着许多特定的膜和细胞质变化。利用简单的组织培养技术，已能够建立一个与体内情况非常相似的角质形成细胞分化体外模型。形成了一个向表面成熟的多层结构。表层细胞的特征是质膜增厚、张力丝浓度增加、细胞间黏附丧失以及存在膜包颗粒。结论是，这是一个适用于正常和异常口腔上皮生物学和病理学研究的体外模型。
