Landay M A, Schroeder H E
J Anat. 1979 Jan;128(Pt 1):31-51.
The epithelium of normal human buccal mucosa was studied by light and electron microscopy and stereological procedures in twenty punch biopsies of 10-15 years old females. Morphometric point counting techniques were employed in order to estimate the density of cell membranes in the superficial third of this epithelium. Observations and data suggested that (1) there is a dense and regular papillary body of slender connective tissue papillae which bifurcate within and penetrate the basal two thirds of the epithelium; (2) the epithelium comprises two major cell compartments, namely (a) the formative and proliferating basal and suprabasal cells, and (b) the differentiated but maturing spinous, and the subsurface and surface cells; (3) the major step in differentiation occurs at the boundary between suprabasal and lower spinous cells; (4) cytoplasmic composition is a reliable indicator of differentiation, which occurs prior to cell flattening; and the relative density of filamentous networks is an indicator of maturation; while cell flattening may be the sign of adaptation to functional needs, i.e. distensibility.
采用光镜、电镜及体视学方法，对20例10 - 15岁女性颊黏膜穿刺活检组织的正常人体颊黏膜上皮进行了研究。运用形态计量学的点计数技术，以估计该上皮表层三分之一区域细胞膜的密度。观察结果和数据表明：（1）存在一个密集且规则的乳头体，由细长的结缔组织乳头组成，这些乳头在基底层三分之二的上皮内分支并穿透该区域；（2）上皮由两个主要细胞区室组成，即（a）形成性和增殖性的基底细胞和基底上层细胞，以及（b）分化但仍在成熟的棘层细胞、棘层下层细胞和表层细胞；（3）分化的主要步骤发生在基底上层细胞和棘层下层细胞之间的边界处；（4）细胞质组成是分化的可靠指标，分化发生在细胞扁平化之前；丝状网络的相对密度是成熟的指标；而细胞扁平化可能是适应功能需求（即可扩张性）的标志。
